Research on the Promotion of Body Length Growth by Increasing Adenosine Expression in Animal Brain Tissue with Bupleurum Extract

Yin Na; Liang Guohui; Zhang Yunfang

doi:10.3969/j.issn.1004-5775.2026.02.004

Abstract：Objective:To explore the impact of Bupleurum extract on the expression of adenosine in animal brain tissue and growth in body length.Methods:Thirty mice were randomly divided into a blank control group(deionized water),a Bupleurum extract group(Bupleurum extract aqueous solution at 0.470 mg/g),and a positive control group(Jujube seed saponin A aqueous solution at 0.013 mg/g),with 10 mice in each group,gavage once a day for 25 days.After the intervention,the body lengths of the mice in each group were measured,and the levels of growth hormone(GH)in the serum and adenosine(AP)in the brain tissue were determined by enzyme-linked immunosorbent assay(ELISA).Twenty-four rats were randomly divided into a blank control group(deionized water),a Bupleurum extract group(Bupleurum extract aqueous solution 0.330 mg/g),and a positive control group(Jujube seed saponin A aqueous solution 0.009 mg/g),with 8 rats in each group,gavage once daily for 3 days.Sleep status of the rats in each group was measured using Xiaomi bracelets,and the levels of GH in the serum and the AD and adenosine triphosphate(ATP)in the preoptic area of the hypothalamus were determined by ELISA.Sixty mice were randomly divided into a blank control group(deionized water),a Bupleurum extract group(Bupleurum extract aqueous solution 0.470 mg/g),an adenosine group(intraperitoneal injection of adenosine aqueous solution 0.280 mg/g),A1R group,A2AR group,and A3R group(Bupleurum extract aqueous solution 0.470 mg/g,simultaneously intraperitoneally injected with DPCPX 2 mg/kg,SCH58261 1 mg/kg,MRS-1523 1 mg/kg respectively),with 10 mice in each group,intervened once a day for 10 days.The levels of GH in the serum and the AD and ATP in the brain tissue were determined by ELISA.Results:Bupleurum extract could significantly increase the body length of mice,elevate the levels of serum GH and brain tissue AD,extend the duration of slow-wave sleep and total sleep time in rats,and increase the levels of serum GH and brain tissue AD and ATP in rats,and the difference was statistically significant(P＜0.05).A1 receptor antagonists could counteract the increase in AD and ATP content in mouse brain tissue caused by Bupleurum extract,A2A receptor antagonists could counteract the increase in GH,AD,and ATP levels simultaneously,and the difference was statistically significant(P＜0.05);A3 receptor antagonists have no significant effect on the indicators,and the difference were not statistically significant(P＞0.05).Conclusion:Bupleurum extract could promote the growth in body length of animals,and its mechanism of action might be related to the increased release of AD and ATP in brain tissue,activation of A1 and A2A receptors,extension of slow-wave sleep duration in animals,and promotion of GH secretion.