Effect of Digitoxin on the Expression of NIS Protein in Thyroid Papillary Carcinoma Cells
Wang Dan
Ma Jinling
Duan Binhong
Abstract：Objective:To investigate the effect of digitoxin on the expression of sodium-iodine symporter(NIS)protein in thyroid papillary carcinoma(PTC)cells.Methods:Different concentrations of digoxigenin solution were prepared,and the concentration of digitoxin solution was 0.25umol/L,the concentration of rapamycin was 50 ug/mL,and the concentration of 3-methyladenine(3-MA)was 0.2mmol/L for subsequent experiments.The thyroid papillary carcinoma cell line BC-PAP was divided into a blank control group without any intervention,digitoxin as the experimental group,rapamycin as the positive control group,and digitoxin+3-MA was negative control group.The expression of NIS,autophagy-related protein microtubule-associated protein 1 light chain 3(MAP1LC3)and BECLIN 1 autophagy related protein(Beclin-1)in each group were detected by western blotting.The formation of autophagosomes was observed under transmission electron microscope.Results:Western blot showed that the relative expression of NIS,Beclin-1 and MAP1LC3 protein in digitoxin group was higher than that in control group,with statistically significant differences(P＜0.01).The relative expression of NIS and Beclin-1 protein in rapamycin group was higher than that in control group,with statistically significant differences(P＜0.01),the relative expression level of MAP1LC3 protein was higher than that of the control group,with statistically significant differences(P＜0.01).The relative expression of NIS,Beclin-1 and MAP1LC3 was higher in the digitoxin group than in the rapamycin group,with statistically significant differences(P＜0.01).The NIS,Beclin-1 and MAP1LC3 proteins in the digitoxin+3MA group were relative.The expression level was lower than that of the digitoxin group,with statistically significant differences(P＜0.01).Conclusion:Digitoxin can increase the expression of NIS protein in thyroid papillary carcinoma cells,and its mechanism may be related to the induction of autophagy.
Keywords：DigitoxinThyroid papillary carcinomaNIS protein
Publication Date：2026-01-25
Online Publishing Date：2026-03-23(First online date of this platform, not the publication date of the document)
Pages：4( 131-134 )