Expression and Clinical Significance of miR-224 in Acute Lymphoblastic Leukemia
Yan Xiaolin
Peng Peng
Guo Ping
Abstract：Objective:To investigate the expression level and clinical significance of MicroRNA(miR)-224 in peripheral blood mononuclear cell(PBMC)of patients with acute lymphoblastic leukemia(ALL),aiming to provide novel biomarkers and therapeutic targets for the diagnosis,prognosis assessment,and treatment of ALL.Methods:From January 2023 to June 2024,a total of 40 adult patients with newly diagnosed ALL in Heilongjiang University of Traditional Chinese Medicine were enrolled as the case group,and 10 healthy adult volunteers served as the control group.PBMC were isolated using density gradient centrifugation,and the relative expression of miR-224 was detected by quantitative real-time polymerase chain reaction(PCR).Clinical data,including gender,age,blood cell counts,and chromosomal karyotypes were collected.Patients were followed up to analyze the relationship between miR-224 expression and the clinical characteristics and prognosis of ALL.Results:The expression level of miR-224 in PBMCs of patients was significantly higher than that in the control group the difference was statistically significant(t=12.12,P＜0.05).Correlation analysis between miR-224 expression and clinical characteristics revealed that high miR-224 expression was not associated with general demographic features such as age and gender the difference was not statistically signficant(P＞0.05).However,it was associated positively with high peripheral blood leukocyte number(r=0.694 9),high bone marrow tumor burden,abnormal chromosome karyotype and poor prognosis in ALL patients with high miR-224 expression the difference was statistically significant(t=4.890,10.410,4.812;P＜0.05).Conclusion:High expression of miR-224 in ALL patients is associated with increased peripheral white blood cell counts,high bone marrow tumor burden,abnormal chromosomal karyotypes,and poor prognosis.miR-224 may contribute to the dysregulation of post-transcriptional control by modulating ALL-related tumor suppressor genes or oncogenes,leading to abnormal proliferation of ALL.Therefore,high expression of mir-224 can be used as a marber for diagrosis and prognosis monitoring of ALL patients.
Keywords：Acute lymphoblastic leukemiaMicroRNA-224Peripheral blood mononuclear cell
Publication Date：2025-10-10
Online Publishing Date：2025-11-05(First online date of this platform, not the publication date of the document)
Pages：4( 2356-2358,2362 )