Comparative Analysis of Colloidal Gold Method and Enzyme-linked Immunosorbent Assay for Detection of AIDS Virus(HIV)Antibody
Guo Xinfang
Abstract：Objective:To test the role of colloidal gold method and enzyme-linked immunoassay(ELISA)in HIV.Methods:133 blood samples from the center were positive for HIV screening.All blood samples were divided into group A(48),B(40)and C(45).Group A was tested by the colloidal gold method twice,Group B was tested by the ELISA twice,and Group C was tested by the colloidal gold method and the ELISA respectively.Protein printing(Western Blot,WB)was used as the gold standard to calculate diagnostic sensitivity,specificity and compliance rate.Results:Kappa value=0.37 for colloidal gold method.Kappa value for ELISA secondary check for HIV was 0.41.Kappa value=0.73 for colloidal gold method and ELISA.The diagnostic sensitivity,specificity,positive predictive value,negative predictive value and accuracy were 83.33%,66.67%,94.59%,36.36%and 81.25%.In group B,the diagnostic sensitivity,specificity and accuracy were 88.58%,60.00%,93.94%,42.86%and 85.00%.The diagnostic sensitivity,specificity,positive predictive value,negative predictive value and accuracy were 94.87%,83.33%,97.37%,71.43%and 93.33%.Conclusion:The application of colloidal gold method and ELISA re-examination for HIV antibody detection has a relatively high diagnostic efficacy.
Keywords：AIDS virusEnzyme linked immunosorbent assayColloidal gold methodInspectionAccuracy
Publication Date：2025-09-10
Online Publishing Date：2025-10-09(First online date of this platform, not the publication date of the document)
Pages：4( 2108-2110,2113 )