Experimental in Vitro Study of Bitter Ginseng Decoction in Combination with Miconazole Nitrate Against a Clinical Strain of Candida Albicans VVC

Xue Shicong; Lin Lichan; Yang Ying

doi:10.3969/j.issn.1004-5775.2025.05.013

Abstract：Objective:To investigate whether bitter ginseng decoction and 2％miconazole nitrate solution act synergistically to enhance their antimicrobial effects against Candida albicans.Methods:Vulvovaginal secretions were collected from patients suffering from vulvovaginal Candida infections,and 10 Candida albicans strains were isolated and cultured,and identified.The minimum inhibitory concentration(MIC)of the decoction of Panax quinquefolium and 2％miconazole nitrate solution against the 10 Candida albicans strains was analyzed and compared with that of the combined solution.And their combined inhibitory indices(FICI)were calculated with reference to the microbial dilution method to determine the effect of the combination of decoction of Panax quinquefolium and 2％miconazole nitrate solution.Results:The mean MIC value of 2％miconazole nitrate solution against Candida albicans was(1.05±0.52)μg/mL.The mean MIC value of bitter ginseng decoction against Candida albicans was(81.25±30.23)mg/mL.The mean MIC value of 2％miconazole nitrate solution against Candida albicans was(0.33±0.23)μg/mdL when bitter ginseng decoction was used in combination with 2％miconazole nitrate.The mean MIC value of anti-Candida albicans of bitter ginseng decoction was(0.51±0.34)mg/mL.The FICI value of bitter ginseng decoction and 2％miconazole nitrate co-administered against Candida albicans was 0.32,which showed synergistic effect.Conclusion:In in vitro experiments,it was found that both the decoction of bitter ginseng and 2％miconazole nitrate solution exhibited stable antibacterial properties against Candida albicans.When these two were used together,they showed significant synergistic effects against Candida albicans.