Role of TM6SF1 Gene in Lung Adenocarcinoma Cells
Liu Tingjun
Abstract：Objective:To investigate the role of TM6SF1 gene in lung adenocarcinoma cells.Methods:The expression of TM6SF1 gene in lung adenocarcinoma and normal tissues was analyzed by GEPIA database.Cell proliferation assay was performed to detect the proliferation of A549 cells after overexpression of TM6SF1.Wound-healing?assay was used to test the speed of wound healing after over-expressing TM6SF1.Transwell cell assay to detect A549 cell invasion and migration after overexpression of TM6SF1.Results:Analysis of the GEPIA database revealed that the expression of TM6SF1 was reduced in lung adenocarcinoma compared with normal tissues,with statistically significant difference(*P＜0.05,**P＜0.01,***P＜0.001),and the survival was longer in the group with high expression of TM6SF1.TM6SF1 negatively correlated with the cell proliferation-associated nuclear antigen Ki-67.After overexpression of TM6SF1,the proliferation,invasion and migration of A549 cells were inhibited,with statisti-cally significant difference(*P＜0.05,**P＜0.01,***P＜0.001),and the expression of PI3K/Akt pathway-associated kinases was down-regulated.Conclusion:The TM6SF1 gene may act as an oncogenic factor in lung adenocarcinoma.
Keywords：Lung adenocarcinoma cellsTM6SF1geneProliferationMigrationInvasion
Publication Date：2023-12-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2837-2840 )