Prognostic and Immune Infiltration Correlation Analysis of Salt-induced Kinase 3 Gene Expression in Human Tu-mors

Chen Yaming; Su Huilin; Zeng Yuanning

doi:10.3969/j.issn.1004-5775.2023.23.002

Abstract：Objective:By analyzing salt-induced kinase 3(SIK3)pan-cancer in human tumors,to explore the expression and prognosis of SIK3 gene in different tumors,and to search for potential targets and tumor biomarkers for tumor immunotherapy.Methods:Differences in the expression of SIK3 gene and protein in normal and tumor tissues were retrieved from the TIMER data-base.The cBioportal tool was used to study SIK3 gene variants in each tumor,and Kaplan-Meier survival curve analysis was per-formed to assess the prognostic value of pan-cancer.Correlation analysis between SIK3 and tumor-associated fibroblast infiltration in TGCA was performed according to EPIC,MCPCOUNTER,and TIDE algorithms.Results:SIK3 was lowly expressed in 13 can-cers,including bladder uroepithelial carcinoma(BLCA),breast cancer(BRCA),and colon adenocarcinoma(COAD).By analyzing the relationship between SIK3 expression and immune-infiltrating T cells in the tumor microenvironment,it was found that the SIK3 expression level was positively correlated with the level of cancer-associated fibroblast infiltration in COAD,head and neck squamous cell carcinoma(HNSC),HPV-infected head and neck squamous carcinoma(HNSC-HPV+),pancreatic adenocarcinoma(PAAD),rectal adenocarcinoma(READ),gastric adenocarcinoma(STAD),testicular germ cell tumor(TGCT),and thymic adenocarci-noma(THYM),and that it was negatively correlated with the level of cancer-associated fibroblast infiltration in low-grade gliomas(LGG)of brain.Pan-cancer survival analysis showed that SIK3 high-expression had a poorer prognosis in adrenocortical carcinoma(ACC),and SIK3 low-expression had a poorer prognosis in renal clear cell carcinoma(KIRC),LGG,mesothelioma(MESO),PAAD,and thyroid cancer(THCA).Conclusion:The SIK3 gene plays an important role in the tumor immune microenvironment and has been identified as having prognostic value in a variety of cancers,speculating that the SIK3 gene is a potential target for cancer im-munotherapy and tumor biomarkers.