Change of DKK1 mRNA in the Process of Epilepto genesis in the Kainate-induced Epilepsy Model

YU Bin; XUX iang-ping; GAO Ying

doi:10.3969/j.issn.1004-5775.2016.10.001

Abstract：Obje ctive To detect the expression of DKK1,an inhibitor of Wnt/β -catenin signaling pathway,in the formation of tempo-ral lobe epilepsy in rats with kainite -induced seizures and to explore the effects of the valproate on the gene expression of DKK1mRNA.Methods Sprague -Dawley rats were induced by kainic acid (KA) at postnatal 30 days, and Real -time PCR method was applied to detect the expression of DKK1 mRNA in the hippocampus in one week,4 weeks and 8 weeks after KA kindling,meanwhile wheth-er VPA could affect the expression of DKK1 mRNA in the hippocampus during the epileptogensis was investigated.Results After KA -in-duced epilepsy occurred,the expression of hippocampal tissue DKK1 mRNA fell significantly (0.42 ±0.17, P =0.000) compared with NS group (1.00 ±0.00) during the acute stage in the formation of temporal lobe epilepsy.At the initial stage,DKK1 mRNA in the hippocampus increased (1.04 ±0.24,P =0.896), but the difference was not statistically significant.While the expression during the stable formation of chronic epilepsy was higher (1.35 ±0.25, P values respectively are 0.000,0.045) than that of the previous two periods with statistic sig-nificance.The expression of hippocampal tissue DKK1 mRNA in the kainate +valproate (KA +VPA) group,compared with NS group, dropped significantly (0.65 ±0.26, P =0.008).But the expression elevates at chronic stage (1.30 ±0.31, P =0.039), and the differ-ence has statistical significance.The expression during the chronic phase was higher than that of the acute phase (0.65 ±0.26), and the early formation of epilepsy (0.94 ±0.18) had statistical significance (1.30 ±0.31, P respectively values were 0.003,0.033).At different stages of epileptogenesis,Wnt5a mRNA expression in the hippocampus had no significant differences between KA group and KA +VPA group.Conclusion DKk1 may participate in the formation of temporal lobe epilepsy by inhibiting the Wnt /β-catenin signaling path-way.The antiepileptic mechanism of valproate acid is not related to DKK1 in this epileptic model.