Experimental Study of Using Anhydroicaritin and Bone Marrow Mesenchymal Stem Cells to Repair the Critical-sized Cranial Bony Defect in Nude Mice
ZHAO Shu-yan
MA Ze-yun
Abstract：Objective To investigate the effects of anhydroicaritin on the repair of the critical -sized cranial bony defect in nude mice . Methods Bone marrow mesenchymal stem cells (BMSCs) were cultured and identified by flow cytometry .Critical-sized cranial bone de-fect was created , then filled with alginate hydrogel ( blank control group ) ,anhydroicaritin in alginate hydrogel ,or anhydroicaritin plus BMSCs in alginate hydroge .All animals were scarified at 2 months post-transplantation for micro-CT examination.Results The cultured BMSCs were characterized as CD73 +CD90 +CD34 -CD45 -cells.Micro -CT results showed that compared with blank control group , newly -formed bone was slightly but significantly increased in anhydroicaritin group , and markedly increased in anhydroicaritin plus BMSCs group (P<0.01).Conclusion Anhydroicaritin could promote the repair of bone defect in vivo , and the repair ability could be significantly en-hanced when anhydroicaritin is used in combination with BMSCs .
Keywords：AnhydroicaritinBMSCsCritical-sized cranial bony defect
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 490-492 )