Rapidity and Accuracy Research of Laboratory Studies of Three Kinds of Methods for Identification of Clostridium Difficile

WEI Xue-wu; ZHANG Zhi-hong; XU Gui-biao

doi:10.3969/j.issn.1004-5775.2015.10.025

Abstract：Objective To discuss the rapidity and accuracy of laboratory studies of three kinds of methods for identification of Clostridium difficile.Methods Selecting 118 ICU patients from April , 2014 to December , 2014.A total of 234 specimens were collected to conduct 24h and 48h culture studies.Then the results were identified and cultured , followed by a method of genetic testing stool samples DNA was extracted from B toxin detection.Then 48h culture samples test results were as reference value of 24h culture assays and Pcr toxin B gene amplification assay was done to evaluate the efficacy , main specificity , sensitivity , negative predictive value and positive predictive value.Results All specimens were cultured after 48h.Among 9 patients, a total of 10 copies of Clostridium difficile specimen culture were posi-tive, and the specimens positive rate was 4.27%.Clostridium infection rate was 7.62%.24h culture of effectiveness ratios were as follows:76.00%sensitivity and 100.00%specificity, 100.00%negative predictive value and 97.00%positive predictive value.Pcr efficacy tcdB gene amplification assay ratios were as follows:82.30%sensitivity and 99.15%specificity degree , 99.15%negative predictive value and 82.30%positive predictive value.Conclusion Pcr toxin B gene amplification assay has the shortest time , sensitivity and specificity are relatively high, 24h culture sensitivity is slightly lower , and it may lead to misdiagnosis;48h culture has the longest time , but it has the most reliable test results.