Effect of Bosentan on Collagen Deposition and MMP-9/TIMP-1 of Pulmonary Fibrosis

doi:10.3969/j.issn.1004-5775.2015.06.002

Abstract：Objective To observe the effect of bosentan on collagen deposition and MMP -9/TIMP-1 of pulmonary fibrosis (PF). Methods 48maleadultWistarratswererandomlyandequallydividedintocontrolgroups(C2,C4group),modelgroups(M2,M4group) and treated groups(T1, T2 group).Bleomycin was used to create PF models.The collagen deposition in lung was observed by Masson′s tri-chrome stain, and the level of MMP-9 and TIMP-1 in BALF were measured by ELISA.Results (1)There was less collagen deposition in C2 and C4 groups.Light blue collagen deposition could be seen in M 2 group.M4 group showed significant serious fibrosis .T1 group was significantly less than M4 group, and there was no significant difference between T 2 and M4.(2)MMP-9 expression was increased in C2, C4, M2 and M4 groups [(1.15 ±0.27),(1.23 ±0.31), (1.98 ±0.35)、(2.38 ±0.47) ng/mL] , which were significantly lower than in T1 group than M4.The level of TIMP-1 was higher in M2 and M4 groups(P<0.05).(3) TIMP-1 in M2 and M4 groups was signifi-cantly higher than C2, C4 and T1 groups, and significantly lower than M4 group.There was statistical significance.Conclusion Bosentan can alleviate the collagen deposition in lung of pulmonary fibrosis in rats , and inhibit the expressions of MMP -9/TIMP-1 in early stage.