The Influence of NADH on the Activity of PARP and Caspase 3 after the Normal Hepatocyte L02 Being Irradiated by X-ray
LIU Fa-quan
ZHANG Ji-ren
Abstract：Objective To study the influence of NADH on cell apoptosis induced by X-ray and the activity of PARP and Caspase 3. Methods This experiment established the pseudo-irradiation group, control group and treatment group.RPMI 1640 medium with and without NADH(400μg/mL)was respectively added to the normal hepatocyte of the control group and treatment group after the normal hepa-tocyte was irradiated under 6MV varian 5.0Gy.The normal hepatocyte was cultured for 24hours.The apoptosis rate was tested by Annexin V/PI, bcl-2 and Bax protein positive expression rate were tested by flow cytometry, the activity of poly-ADP-ribose polymerase PARP and Caspase3 were tested by western blot.Results Comparing with the control group, the apoptosis rate obviously reduced, Bax protein positive expression percentage apparently reduced but the bcl-2 expression percentage obviously increased with ( P<0.05 ) .Both the PARP and Caspase 3 of the control group did not emerge cut fragment and the treatment group did not find the cut fragment.Conclusion NADH can resist the damage and apoptosis of L02 normal hepatocyte reduced by the radiation.Its mode of action may be associated with the mitochondria of bcl-2 and bax.
Keywords：Nicotinamide Adenine Dinucleotide HydrogenRadiation protectionApoptosisMitochondria
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 468-469 )