Effect of Gemcitabine on Expression of Inhibition of Mammary Tumor Cells in Vitro and its MTA1 mRNA

CHENG Hai-ying; ZHENG Ding-rong

doi:10.3969/j.issn.1004-5775.2014.11.005

Abstract：Objective To investigate the inhibitory effect and mechanism of action of gemcitabine on breast cancer cell line MCF-7 in vitro.Methods The concentrations of 25, 50, 100, 200 and 400mg/ml gemcitabine culture were divided into five groups: A, B, C, D and E groups.A, B, C, D and E groups were in vitro culture of logarithmic growth phase of breast tumor cell line MCF-7 for 24h;methyl thiazolyl tetrazolium ( MTT) assay was used for the detection of 5 groups of breast tumor cell line MCF-7 growth inhibition rate;real-time quantitative polymerase chain reaction ( PCR) was used to detect the relative expression of mRNA of breast cancer MCF-7 cell line metas-tasis associated gene 1 (MTA1) in 5 groups.Results In A, B, C, D and E in breast cancer cell line MCF-7 inhibition rate of A group<B group <C group <D group <E group, the differences were statistically significant (P<0.05);the relative expressions of C group, D group and E group MTA1 mRNA were lower than the blank control group.MTA1 mRNA relative expressions quantity of the three groups reduced, and the differences were statistically significant ( P <0.01).Conclusion Gemcitabine can inhibit the proliferation of breast cancer cell line MCF-7, and has dose dependent.Its main mechanism inhibits breast cancer cell line MCF-7 and the role of anti breast cancer metastasis through the down-regulation of MTA1 mRNA expression.