Bacterial Community Succession Pattern in Wastewater Plant Containing Two-layer CRI
MENG Xu
XING Tao
TONG Hongjin
ZHANG Yunjie
Yong Yi
HOU Jiang
LIN Kang
LIN Rui
WU Di
Abstract：The artificial rapid infiltration system(CRI)is widely used for advanced treatment of wastewater in sewage plants.The connectivity between CRI and various front-end treatment units allows for the variation of bacteria along the process,which can be influenced by environmental conditions.However,there is currently a lack of relevant research to scientifically guide the addition of fortified bacteria.agents.Therefore,this study focuses on the microbial community along each unit of a sewage plant and investigates the patterns of microbial community changes using high-throughput sequencing.The results show that high concentrations of wastewater and dissolved oxygen will reduce both the abundance and diversity of microbial communities,but the proportion of dominant bacteria with abundance greater than 5％in the microbial communities shows an opposite trend.Similar trends are also observed in different depths within the CRI system itself.In the front-end processing unit,approximately 62.2％of bacterial species can enter CRI-1,while CRI-1 itself can differentiate into 37.8％of bacterial species,CRI-2 further generates a differentiation of 17.4％of bacterial species.some dominant bacteria exhibit clear patterns.Thauera,norank_f_Anaerolineaceae and unclassified_f_Comamonadaceae are stably present in all units but with higher abun-dance for Thauera and norank_f_Anaerolineaceae.Ottowia rapidly decreases in abundance as it enters into CRI while norank_f_Synergistaceae decreases slowly.Truepera rapidly increases in abundance as it enters into CRI while nonrank_c_Ac-idobacteria and nonrank_f_Nitrosomonadaceae increase slowly.The paper emphasizes that targeted addition of bacterial agents should be made based on the evolutionary trends of the microbial community along the way during the inoculation and colonization activation.
Keywords：high-throughput sequencingCRIbacterial abundancediversity indexfortified bacteria
Publication Date：2024-12-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 105-114 )