Research on the Functional Characteristics of Microbial Communities in Landfills in Relation to Landfill Time
LI Huikang
WANG Jing
CHENG Zhaowen
WANG Hui
HUANG Qiujie
YANG Changfu
WANG Luochun
NING Chengqi
LOU Ziyang
Abstract：Microbes play a central role in the stabilization process of domestic waste landfills.However,the succession of microbial communities and their metabolic functional characteristics at different stabilization stages remained rare,directly affecting the scientific choice of regulation methods.The research investigated the diversity of microbial communities and metabolic characteristics of functional gene genera using 16S rRNA high-throughput sequencing technology for refuses at different disposal ages.The results showed that the microbial community composition was related to the ages of the landfill,and principal coordinate analysis(PCoA)explained 26.26%and 12.04%of the changes in microbial community abundance,respectively,and the bacterial community composition of the refuses with a disposal ages<10 years showed a significant difference from the other samples.Microbial alpha diversity decreased with increasing disposal age.Firmicutes(32.1%～64.1%)and Proteobacteria(28.6%～53.3%)were the dominant phyla in the landfill,and the proportion of Firmicutes increased with the increase of disposal ages as it promoted the degradation of macromolecular organic matter.The relative abundance of Proteobacteria,as a phylum that decomposes soluble substrates,tended to decrease due to the gradual decrease of easily degradable organic matter with the increase of disposal ages.The functional gene genera in landfill were diverse,with 4 sulfate-reducing genera,3 genera involved in the nitrogen cycle,6 functional genera involved in methanogenic processes,1 organic pollutant-degrading bacterium,6 cellulolytic genera and 13 plastic-degrading microorganisms found.The metabo-lism pathway in KEGG Level 1 was the most abundant metabolic function.Amino acid metabolism,carbohydrate metabolism,energy metabolism and metabolism of cofactors and vitamins were the relatively abundant metabolic pathways in level 2.RDA redundancy analyses indicated that BDM,OM,pH,C,L,and moisture were important environmental factors driving microbial diversity in the landfill.
Keywords：landfillmicrobial diversityKEGG metabolic function16S rRNA
Publication Date：2023-11-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 20-29 )