Effect of Fe2+ and Fe3+ on N2O Emission of Anammox System
YANG Yudifei
SHEN Yanwen
ZHU Nanwen
Abstract：Some studies have shown that appropriate Fe2+ and Fe3+ can promote the metabolism of anammox bacteria,but the effect on the N2O production of bacteria is still unknown.In this paper,a batch experiment was conducted to investigate the N2O production of anammox at different concentrations of Fe2+ and Fe3+,and analyze its microbiome and functional genes.The results showed the highest N2O(0.29 mg/L)in the Fe2+ group was at 20 mg/L Fe2+,and decreased by 47.01%at 30 mg/L Fe2+.In the Fe3+ group,the N2O emission increases with the Fe3+ concentration,and the maximum N2O emission is 0.17 mg/L at 30 mg/L Fe3+.Microbial community diversity analysis showed that 5 mg/L Fe2+ and Fe3+ could stimulate the growth of Candidatus Kuenenia,but denitrification bacteria Denitratisoma and Limnobacter increased with the increase of Fe2+ and Fe3+ concentrations.Functional gene analysis showed that the release of N2O was related to functional genes in nitrification pathway,DNRA pathway,denitrification pathway and anammox pathway,and was significantly positively correlated with genes norB,nirD and hcp(P<0.05).This paper describes the effects of Fe2+ and Fe3+ on N2O emission in anammox system,which can provide reference for N2O control in anammox process.
Keywords：Fe2+Fe3+N2Oanammoxmicrobial communityfunctional genes
Publication Date：2023-11-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 11-19 )