Effects of Sulfur Sources on Nitrogen Removal Performance and Microbial Community Structure of Autotrophic Denitrification Process
LI Zhenghui
LIU Baohe
YU Haoran
MENG Guanhua
TONG Yangyang
ZHANG Huijuan
JIANG Yongbin
Abstract：In this study,denitrification performance of sulfur autotrophic denitrification system driven by different sulfur sources and their respective advantages and disadvantages was systematically explored.An upflow anaerobic sludge blanket reactor was used to study the effects of concentration and hydraulic residence time of influent NO3--N on the denitrification performance of supported elemental sulfur(PFSF)autotrophic denitrification system and sodium thiosulfate(Na2S2O3)autotro-phic denitrification system.The synergistic competition between the two systems was analyzed from the perspective of micro-bial community structure.The results showed that under the same conditions,the nitrogen removal performance,load resistance,and stability of Na2S2O3 autotrophic denitrification system were superior to those of PFSF autotrophic denitrifica-tion system.The dominant bacteria in both systems were Thiobacillus and Sulfurimonas,and the abundance of autotrophic denitrifying bacteria in the two systems was 49.92％and 47.54％,respectively.The microbial community structure in Na2S2O3 system was more complex than that in PFSF system.Na2S2O3 system and PFSF system exhibited a stable microecological balance.A synergistic effect was observed among autotrophic denitrifying bacteria and mixotrophic denitrifying bacteria in Na2S2O3 system,and a synergistic effect was observed among autotrophic denitrifying bacteria and anammox bacteria in PFSF system,as well as an interspecific sulfur cycle process was found in PFSF system.Based on cost analysis,the economic value of PFSF system was found to be better than that of Na2S2O3 system.The results showed that both autotrophic denitrification systems with Na2S2O3 and PFSF as electron donors exhibited good nitrogen removal performance and the PFSF system was found to be more economical.This study provides theoretical support for the application of elemental sulfur autotrophic deni-trification for nitrogen removal in practice.
Keywords：sulfur autotrophic denitrificationelemental sulfur fillerthiosulfatemicrobial community synergyeconomic analysis
Publication Date：2023-09-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 1-10 )