Screening of Mutant Strains Capable to Metabolize High Concentrations of Formaldehyde Using Atmospheric and Room Temperature Plasmas
KONG Fang
GAO Yong
CAI Weirong
ZHAO Xiang
WANG Youping
Abstract：Formaldehyde(FD) has strong cytotoxicity to the organism and is seriously harmful to human health.So microbial degradation has become one of the most effective way for the removal of formaldehyde.The purpose of this study was to obtain mutants capable to metabolize high concentration of formaldehyde in existing strains.Atmospheric and room temperature plasmas (ARTP) mutagenesis system can help to cause high mutation rate and wide mutational spectrum with lower damage.Taking the strain Pseudomonas putida Wl as the original strain,mutants were quickly obtained by using ARTP mutation and then compared them with gradient plate screening method.The results showed that mutant WMZ120-5 in the solid media culture could tolerate maximum concentrations of formaldehyde ranging from 10 g/L to 20 g/L,showing more than 10 times higher compared with those by the wild-type Wl under the same conditions.Formaldehyde removal efficiencies above 98％ in 96 h was obtained at formaldehyde concentration equal to or less than 3 g/L.Its character was stable after seven generatations.And optimal parameters for 4 g/L formaldehyde-degrading condition was initial pH 7.5,30 ℃ and 5％ inoculum dose.More than 95％ formaldehyde was consumed within 168 h under optimized conditions.The results showed that mutant WMZ120-5 has a good tolerance to formaldehyde and high degradation efficiency to formaldehyde,and potential use for remediation of formaldehyde pollution.
Keywords：Pseudomonasformaldehydedegradation characteristicsatmospheric and room temperature plasmas (ARTP)gradient plate screening
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 95-102 )