Isolation, Identification and Characterization of a Decolorization Strain for Azo Dye Reactive Black 5
ZHANG Chongmiao
WANG Dai
MOU Xiao
LIU Qiangqiang
MA Yuchao
JIN Pengkang
Abstract：A bacterial strain,EWA2,with remarkable ability to decolorize azo dye Reactive Black 5,was isolated from activated sludge collected from a wastewater treatment plant in Xi'an.By means of biochemical test and homology comparison of 16S rDNA sequence,strain EWA2 was identified as Proteus mirabilis.In LB liquid medium,decolofization rate of Reactive Black 5 (≤300 mg/L) by strain EWA2 could roached 94.7％～99.9/％ within 48 hours.In simulated wastewater containing Reactive Black 5,strain EWA2 could utilize sodium carboxymethyl cellulose and glucose as carbon source to significantly increase the decolorization rate.Under the condition in 8％ NaC1,the decolorization rate was 83.6％ within 48 hours.It indicated that biodegradation was the main way of decolorizing Reactive Black 5 by strain EWA2,and the adsorption peak of degradation products appeared in near-ultraviolet region.
Keywords：Reactive Black 5decolorizationisolationidentificationProteus mirabilis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 50-54 )