Physicochemical and Microbial Properties of Citric Acid Wastewater Anaerobic Granular Sludge in Multi-phase Internal Circulating Anaerobic Reactor
ZHENG Xinyuan
DONG Liming
WANG Ping
ZHANG Yanping
LI Yingying
TANG Wentao
Abstract：This study mainly investigated the physicochemical and microbial properties of citric acid wastewater anaerobic granular sludge in multi-phase internal circulating anaerobic reactor.Under the scanning electron microscope,the anaerobic granular sludge looked black and spherize,with gaps on smooth surface.By means of sieving method,it was found that 90％of the granular sludge was between 0.45～2.0 mm.And the settling velocity of granular sludge in this range was more than 50 m/h by gravitaional sedimentation,which showed the good sedimentation ability of the granular sludge.After calcination at 600 ℃,the volatile of granular sludge measured,accounting for 64.56％ of total solid,indicating the granular sludge contained a lot of organic matter.More than 30 phyla and 100 genera including dominant genera such as Anaerofustis (4.91％),Anaerolineae (4.78％),Spirochaeta (4.41％) and Longilinea (3.98％) were detected from anaerobic granular sludge using the 454 GSFLX pyro-sequencing platform.The common features of the metabolic process of these dominant genra were hydrolyzing macromolecular organic matter into acid.The main function of Methanobacterium (49.35％) and Methanosaeta (7.81％) was producing CH4.The high values of diversity index indicated the comrmunity richness and diversity of microbes in anaerobic granular sludge,which were in favor of the stable operating of the anaerobic reactor.
Keywords：citric acid wastewatermulti-phase internal circulating anaerobic reactoranaerobic granular sludgephysiochemical propertiesmicrobial properties
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 73-77 )