Isolation and Identification of a Stain with High Efficiency Decolorization of Direct Red FR Dye and Its Versatile Characteristics
FENG Lei
GE Su
WU Jing
FANG Yuan
ZHANG Yuan
WU Cong
ZHENG Yongliang
Abstract：A strain HS-M02 was isolated from a dyestuff plant in Huanggang City,Hubei Province.Research indicated that HS-M02 strain could decolorize and degrade direct red FR dye and used it as the sole nitrogen and energy sources.UV-Vis dynamic scanning results showed that direct red FR dye at the concentration of 20 mg/L was completely decolorized within 24 h at the conditions of 37 ℃,pH 7.0.16S rDNA gene of HS-M02 strain was amplified and blasted online,and phylogenetic tree was constructed with Mega5.1 bioinformatics software.Results suggested that HS-M02 strain was as high as 100％ homologous to Enterococcus faecium DSM 20477.Together with morphology and physiological and biochemical tests,HS-M02 strain was indentified to Enterococcus faecium sp.Further research showed that HS-M02 strain could decolorize dyes of reactive black 5,acid red GR,direct red and vat yellow GCN dye with high efficient degradation ability,and the degradation rate was up to 90％ at the concentration of 20 mg/L dye with 24 h respectively.These results indicated that HS-M02 strain is a great degrader with wide characteristics of dyes decolorization and degradation,and it has a potential research and application prospect.
Keywords：direct red FR dyedecolorizedynamic scanning16S rDNAhigh efficiencyversatile characteristics
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 38-42 )