Isolation, Identification and Heavy Metal Tolerance of an Acidophilic Heterotrophic Microorganism
QIU Xiuwen
ZHOU Guixiang
YANG Qiyong
HAN Jinfeng
JIANG Sheng
SHAN Yaoyao
SUN Chao
Abstract：An acidophilic heterotrophic microorganism JJU-2 was isolated from sewage sludge which was collected from the sewage disposal plant.According to its morphology,physiological-biochemical properties and 26S rDNA gene sequence analysis,the strain was identified as Rhodotorulamucilaginosa.The optimal pH of strain was 5 but it could survive in an extremely low pH 1 condition.The optimum growth temperature was 25 ℃ and the highest temperature it can survive was 40 ℃.The strain grew fast after 4 h and came into the logarithmic growth stage until the stable growth stage after 16 h.Resistant experiment to metals showed that JJU-2 strain could resist cadmium,chromium,copper,nickel and lead with 1.5 mmol/L,10 mmol/L,5 mmol/L,2 mmol/L and 20 mmol/L,respectively.The leaching rates of heavy metals (Cu,Ni,Cd and Cr) were 84％,96％,100％ and 71％ with the combined effect of JJU-2 and JJU-1 strains,respectively.The isolation of the strain Rhodotorulamucilaginosa could be widely used in sewage sludge bioleaching of heavy metal and provides basic material to study the resistance mechanism of microorganisms to heavy metal in extreme conditions.
Keywords：acidophilic heterotrophic microorganismsequence analysismetal toleranceRhodotorulamucilaginosa
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 64-69 )