A Broad-spectrum Keratin-degrading Strain: Screening and Degradation Characteristics
DU Dongxia
XU Jun
YIN Hongmei
LIU Biao
WANG Bin
HE Yuelin
Abstract：A high-efficiency keratin-degrading strain (named E-26) was screened by enrichment cultivation and gradient acclimation.The strain was identified as Bacillus cereus on the basis of morphological examination,physiological and biochemical characterization,as well as 16S rDNA sequence and phylogenetic analysis.The laboratory experiment results suggested,in terms of environmental factors and enzymology characteristics,that the best carbon and nitrogen source of E-26 degrading pig hairs were maltose and yeast powder;the optimum reaction condition of degrading pig hairs was:pH 8.0,temperature 40 ℃C inoculum dose 10％ and substrate concentration 1％.Under the above mentioned optirnized condition and fermental cultivation for 10 d,the keratinase activity of E-26 strain could reach 57.8 U/mL,37.4％ higher than initial activity;and the rate of degradation of E-26 strain reached 71.2％,41.9％ higher than the initial degradation rate.
Keywords：keratin-degrading strainBacillus cereusenzymology characteristicsdegrading pig hairs
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 55-59 )