Expression of lncRNA MELTF-AS1 in bladder cancer tissues and its effect on malignant biological behaviors of bladder cancer cells
QI Pan
CUI Liqun
YAN Xiaohan
Abstract：Objective To detect the expression of long non-coding RNA(lncRNA)MELTF-AS1 in bladder cancer(BC)tissues and cells,the relationship between its expression and tumor stage(T stage)of BC patients,and its impact on biological behaviors of BC(e.g.,cell proliferation,migration and invasion).Methods Cancer tissues and corresponding normal bladder urinary tract epithelial tissue adjacent to cancer tissues were surgically collected from 42 BC patients who underwent surgical treatment and confirmed by pathology in the Fourth Hospital of Hebei Medical University from September 2021 to October 2022.The expression of MELTF-AS1 in BC tissues,BC cell lines(5637,T24,SW780)and normal bladder epithelial cell line(SV-HUC-1)was detected by real-time quantitative PCR.Additionally,the relationship between MELTF-AS1 level and clinical parameters in BC patients was analyzed.The overexpression vector pcDNA3.1-MELTF-AS1 and knockdown vector si-MELTF-AS1 were constructed and transfected into 5637,T24,and SW780 cells.The effects of overexpression or knockdown of MELTF-AS1 on the proliferation,migration and invasion of BC cells were detected by MTS assay,colony formation assay,wound healing assay,and Transwell invasion assay.Results The relative expression of MELTF-AS1 in BC tissue was significantly higher than that in its corresponding normal bladder urothelium adjacent to cancer tissue.Compared with non-muscle invasive BC patients,the expression level of MELTF-AS1 in muscle invasive tissues of BC patients was significant higher.Its expression was significantly related to the depth of tumor invasion(T stage).MELTF-AS1 was up-regulated in all three bladder cancer cell lines compared with SV-HUC-1 cells,with the highest expression in 5637 cells.Knockdown of MELTF-AS1 significantly inhibited the proliferation,migration,and invasion abilities of 5637 cells,while overexpression of it significantly promoted the proliferation,migration,and invasion abilities of T24 cells(P<0.05).Conclusion MELTF-AS1 is overexpressed in BC tissues and cells,which can promote the biological behaviors of proliferation,migration and invasion in BC cells.
Keywords：long non-coding RNAMELTF-AS1bladder cancerbiological behavior
Publication Date：2026-02-26
Online Publishing Date：2026-03-23(First online date of this platform, not the publication date of the document)
Pages：6( 217-222 )