Influence of the modified Huangqi Biejia Powder in promoting the apoptosis of CT26 cells by the Bcl-2/Bax/Caspase-3/Caspase-8 signaling pathway
GU Jia
LIU Qiang
SHI Mengjiao
Abstract：Objective To explore the influence of the Astragalus and Turtle shell Powder(modified Huangqi Biejia Powder)on the proliferation and apoptosis of CT26 cells by regulating the B cell lymphoma-2(Bcl-2)/Bcl-2-associated X protein(Bax)/Caspase-3/Caspase-8 signaling pathway.Methods A total of 24 male Sprague-Dawley(SD)rats were randomly divided into the control group,and low-dose,medium-dose and high-dose Huangqi Biejia Powder groups(modified Huangqi Biejia Powder at 6,12 and 24g·kg-1·d-1,respectively),with 6 rats per group.CT26 cells were induced with drug-containing serum.Cell survival and colony formation ability were tested by cell counting kit-8(CCK-8)assay and colony formation assay.Cell cycle progression and apoptosis were detected by flow cytometry.Protein levels of Bcl-2,Bax,Caspase-3 and Caspase-8 were detected by Western blot.Results Compared with the control group,significantly decreased colonies were observed in CT26 cells induced with low-dose,medium-dose and high-dose Huangqi Biejia Powder;significantly decreased survival was observed in CT26 cells induced with medium-dose and high-dose Huangqi Biejia Powder;significantly increased apoptotic rate was observed in CT26 cells induced with high-dose Huangqi Biejia Powder(all P<0.05).Low-dose,medium-dose and high-dose Huangqi Biejia Powder treatment significantly upregulated apoptosis-related proteins,including Bax,Caspase-3 and Caspase-8,but downregulated the oncogene Bcl-2(all P<0.05).The proportion of cells in the G1 phase was significantly reduced in CT26 cells induced with high-dose Huangqi Biejia Powder(P<0.05).Significantly decreased proportion of cells in the G2 phase,and increased proportion of cells in the S phase were observed in CT26 cells induced with low-dose,medium-dose and high-dose Huangqi Biejia Powder(all P<0.05).Conclusion The drug-containing serum of modified Huangqi Biejia Powder significantly inhibits proliferation,induces apoptosis,and causes cell cycle arrest in the S phase of CT26 cells.
Keywords：colorectal cancercell apoptosismodified Huangqi Biejia Powderapoptotic pathwaycell cycle
Publication Date：2026-02-26
Online Publishing Date：2026-03-23(First online date of this platform, not the publication date of the document)
Pages：5( 202-206 )