Effects of dopamine hydrochloride injection combined with hyperbaric oxygen therapy on the emergency treatment outcome,cardiac function and blood glucose metabolism in rats with acute heart failure
JIA Meng
LIU Kui
LAN Fei
Abstract：Objective To investigate the effects of dopamine hydrochloride injection combined with hyperbaric oxygen therapy(HBOT)on the emergency treatment outcome,cardiac function and blood glucose metabolism in rats with acute heart failure.Methods A total of 75 Sprague-Dawley(SD)male rats in the specific pathogen free(SPF)level were randomly divided into sham operation group,model group,dopamine injection group,HBOT group and combination group,with 15 rats per group.An acute heart failure rat model was established in all groups except for the sham operation group.After successful modeling,rats in the dopamine injection group were intraperitoneally injected with 2.0 mg/kg dopamine hydrochloride injection,and HBOT was given to those in the HBOT group.Both dopamine hydrochloride injection and HBOT were given to those in the combination group.The number of deaths,heart rate(HR)and mean arterial pressure(MAP)were recorded.The D700 LAB color ultrasound diagnostic instrument was used to detect cardiac function.Glucose metabolism index was detected by the commercial kit.The pathological morphology of myocardium was observed by hematoxylin-eosin(H&E)staining.The protein levels of p38 mitogen activated protein kinase(p38MAPK),extracellular regulated kinases 1(ERK1)and protein kinase C(PKC)were detected by Western blotting.Results Compared with sham operation group,rats in the model group had significantly higher number of deaths,mean MAP,left ventricular internal dimension at end-diastole(LVIDd),Left ventricular internal dimension at end-systole(LVIDs),glucose(GLU),glycated serum protein(GSP),fasting insulin(FINS),plasma glucose(PG),and protein levels of p38MAPK,ERK1 and PKC,but lower HR,left ventricular ejection fraction(LVEF)and left ventricular fraction shortening(LVFS)(all P<0.05).Compared with model group,rats in the dopamine injection and HBOT groups had significantly lower number of deaths,mean MAP,LVIDd,LVIDs,GLU,GSP,FINS,PG,and protein levels of p38MAPK,ERK1 and PKC,but higher HR,LVEF and LVFS(all P<0.05),but comparable between the latter two groups(P>0.05).Compared with HBOT group,rats in the combination group had significantly lower number of deaths,mean MAP,LVIDd,LVIDs,GLU,GSP,FINS,PG,and protein levels of p38MAPK,ERK1 and PKC,but higher HR,LVEF and LVFS(all P<0.05).Conclusion Dopamine hydrochloride injection combined with HBOT can improve the emergency treatment outcome of acute heart failure rats,improve cardiac function,reduce blood sugar and play a role in cardiac protection.
Keywords：acute heart failuredopamine hydrochloride injectionhyperbaric oxygenpathological morphology of myocardial tissue
Publication Date：2026-02-26
Online Publishing Date：2026-03-23(First online date of this platform, not the publication date of the document)
Pages：5( 192-196 )