Correlation of protein levels of p-4E-BP1 and GLUT1 with the proliferation and invasion of ovarian cancer
WU Juan
QIN Lijuan
LI Wei
Abstract：Objective To investigate the correlation of protein levels of phosphorylated eukaryotic initiating factor 4E binding protein 1(p-4E-BP1)and glucose transporter 1(GLUT1)with the proliferation and invasion of ovarian cancer.Methods A total of 116 ovarian cancer patients diagnosed and treated from January 2019 to July 2020 were selected.Cancer tissue and normal adjacent tissues were intraoperatively collected.Western blot was used to detect protein levels of p-4E-BP1 and GLUT1 in ovarian cancer tissues and normal adjacent tissues.Real-time fluorescence quantitative polymerase chain reaction(RT-PCR)was used to detect the mRNA levels of proliferation-related genes(c-myelocytomatosis oncogene product[c-Myc],Cyclin D1)and invasion-related genes(ITGA2[Integrin Subunit Alpha 2]and CTHRC1[Collagen Triple Helix Repeat Containing 1]).Pearson correlation was used to analyze the correlation between the expression levels of p-4E-BP1 and GLUT1 in ovarian cancer tissue with the expression levels of proliferation and invasion-related genes.The correlation of p-4E-BP1 and GLUT1 levels in ovarian cancer tissue with clinical pathological characteristics was analyzed.Log-Rank test and Kaplan-Meier curve were used to illustrate the 3-year survival of ovarian cancer.Results Compared with normal adjacent tissue,p-4E-BP1 and GLUT1 in ovarian cancer tissue were significantly upregulated(P<0.05).Compared with normal adjacent tissue,the mRNA levels of Cyclin D1,c-Myc,CTHRC1,and ITGA2 in ovarian cancer tissue were significantly higher(P<0.05).Expression levels of p-4E-BP1 and GLUT1 in ovarian cancer tissue were positively correlated with those of Cyclin D1,c-Myc,CTHRC1,and ITGA2 in ovarian cancer tissue(P<0.05).The expression levels of p-4E-BP1 and GLUT1 in patients with FIGO stage Ⅲ-Ⅳ,lymph node metastasis,infiltration depth T3-T4,poorly differentiated tumors were significantly higher than those with FIGO stageⅠ-Ⅱ,no lymph node metastasis,infiltration depth T1-T2,and moderately well differentiated tumors(P<0.05).The 3-year overall survival of ovarian cancer patients with high expressions of p-4E-BP1 and GLUT1 was significantly lower than that of the low expression groups(P<0.05).Conclusion p-4E-BP1 and GLUT1 are upregulated in ovarian cancer tissue,which are related to abnormal expression of proliferation and invasion-related genes,advanced FIGO staging,lymph node metastasis,increased invasion depth,low tumor differentiation,and reduced 3-year overall survival.
Keywords：ovarian cancerphosphorylated eukaryotic initiating factor 4E binding protein 1glucose transporter 1proliferation related genesinvasion related genes
Publication Date：2026-01-26
Online Publishing Date：2026-03-17(First online date of this platform, not the publication date of the document)
Pages：5( 5-9 )