Remifentanil influences the growth of thyroid cancer cells by regulating the miR-21-3p/SMAD7 axis
WANG Yanbing
BI Xiao
LIANG Yanjiao
Abstract：Objective To investigate the role of remifentanil(REM)on the growth of thyroid cancer(TC)cells by regulating the microRNA-21-3p(miR-21-3p)/SMAD family member 7(SMAD7)axis.Methods In vitro cultured TC cells(TPC-1)were treated with REM at varying concentrations ranging from Ong/mL to 500ng/mL,and CCK-8 assay was applied to detect cell viability.The optimal concentration of REM was screened.Cells were induced with blank control,low-dose,medium-dose and high-dose REM,high-dose REM plus transfection of miR-NC or miR-21-3p mimics.Quantitative real-time polymerase chain reaction(QRT-PCR)was applied to detect the expression levels of miR-21-3p and SMAD7 in TPC-1 cells.Cell proliferation and apoptosis were examined by colony formation assay and flow cytometry,respectively.Dua l-luciferase reporter assay was applied to detect the targeting relationship between miR-21-3p and SMAD7.Western blot was applied to detect protein expression of SMAD7.Results REM at concentrations of 5ng/mL,50ng/mL,and 500ng/mL were selected for subsequent experiments.The mRNA level of miR-21-3p in TPC-1 cells was significantly higher than that in NTHY-ORI3.1 cells,and the mRNA level of SMAD7 was significantly lower than that in NTHY-ORI3.1 cells(P＜0.05).Compared with the blank control,low-dose,medium-dose and high-dose REM treatment significantly decreased colony number and miR-21-3p level,but increased apoptotic rate and protein level of SMAD7 in a dose-dependent manner(P＜0.05).Compared with cells treated with high-dose REM and transfected with miR-NC,those induced with high-dose REM and transfected with miR-21-3p mimics had significantly higher colony number and miR-21-3p level,but lower apoptotic rate and protein level of SMAD7(P＜0.05).No significant differences in the colony number,apoptotic rate and expression levels of miR-21-3p and SMAD7 were detected between cells treated with high-dose REM and those treated with high-dose REM and transfected with miR-NC(P＞0.05).Dual-luciferase reporter assay showed that the luciferase activity of WT-SMAD7 in the miR-21-3p mimics group was significantly lower than that in the miR-NC group(P＜0.05).Conclusion REM affects the growth of TC cells by inhibiting the miR-21-3p/SMAD7 axis.
Keywords：thyroid cancerremifentanilmicroRNA-21-3p(miR-21-3p)/SMAD family member 7(SMAD7)axisgrowth
Publication Date：2025-12-26
Online Publishing Date：2026-01-15(First online date of this platform, not the publication date of the document)
Pages：5( 1947-1951 )