LncRNA EBLN3P regulates the proliferation and apoptosis of cisplatin-resistant lung cancer cells by targeting miR-1278

JIAO Bin; DONG Wen

doi:10.3969/j.issn.1002-7386.2025.11.003

Abstract：Objective To investigate the effects of long noncoding RNAs endogenous bornavirus-like nucleoprotein(lncRNA EBLN3P)on the proliferation and apoptosis of cisplatin(DDP)-resistant lung cancer cells H1299/DDP and its molecular mechanism.Methods From June 2020 to June 2023,thirty lung cancer tissues and their paracancerous tissues were selected.The lung cancer cell line H1299 was cultured in vitro,and the DDP-resistant cell line H1299/DDP was established by inducing DDP in a gradient concentration in H1299 cells.Cells were transfected with si-EBLN3P,miR-1278,anti-miR-1278 and corresponding negative controls.The real time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression levels of lncRNA EBLN3P and miR-1278.MTT assay was used to detect cell viability and calculate the half-maximal inhibitory concentration(IC50)value of DDP.Flow cytometry was used to detect cell apoptosis.The expressions of proteins related to cell proliferation and apoptosis were detected by Western blot.Dual luciferase reporter assay was used to detect the targeting relationship between lncRNA EBLN3P and miR-1278.Results Compared with those of paracancerous tissues,lncRNA EBLN3P was upregulated and miR-1278 was downregulated in lung cancer tissues(P＜0.05).The survival rate and IC50 value of H1299/DDP cells treated with varying concentrations of DDP were significantly higher than those of H1299 cells(P＜0.05).Knockdown of lncRNA EBLN3P or overexpression of miR-1278 decreased the optical density(OD),downregulated protein levels of proliferating cell nuclear antigen(PCNA)and B-cell lymphoma-2(Bcl-2),increased apoptotic rate and upregulated protein level of Bcl-2 associated X protein(P＜0.05).LncRNA EBLN3P targeted miR-1278 and regulated its expression level(P＜0.05).Knockdown of miR-1278 reversed the role of silenced lncRNA EBLN3P in inhibiting proliferation and promoting apoptosis of H1299/DDP cells(P＜0.05).Conclusion Knockdown of lncRNA EBLN3P inhibits the proliferation and promotes apoptosis of H1299/DDP cells through a targeted up-regulation of miR-1278.