LncRNA PVT1 regulates the proliferation and apoptosis of the acute myeloid leukemia cell line HL-60 via targeting miRNA-486-5p:a clinical study

AN Hebing; CHEN Shan; WANG Tengfei

doi:10.3969/j.issn.1002-7386.2025.10.021

Abstract：Objective To investigate the effect of knockdown of lncRNA PVT1 on the proliferation and apoptosis of the acute myeloid leukemia(AML)cell line HL-60 via targeting miRNA-486-5p,thus providing a strategy for the treatment and improving the prognosis of AML.Methods A total of 100 AML patients treated in the Second Affiliated Hospital of Hebei North University from April 2019 to March 2023 were analyzed as the study group,and 100 healthy subjects receiving physical examinations during the same period were selected as the control group.Serum lncRNA PVT1 and miR-486-5p were detected by real-time fluorescence quantitative polymerase chain reaction(RT-gPCR),and their correlation was assessed.A total of 100 AML patients in the study group were randomly divided into two groups,and 50 randomly selected patients were included in the transfection group,where HL-60 cells were transfected with lncRNA PVT1 plasmid.The other 50 patients were included in the non-transfection group.Serum lncRNA PVT1 and miR-486-5p in the two groups were detected by RT-qPCR.The proliferation rate of AML cells was detected by cell counting kit-8(CCK-8)assay.The changes in cell cycle and apoptosis were detected by flow cytometry.The expression levels of proliferation-related proteins(Cyclin D1,cyclin-dependent kinase 4[CDK4],proliferating cell nuclear antigen[PCNA])and cell cycle regulators(P21 and P27)were detected by Western blot and RT-qPCR.Results The level of lncRNA PVT1 was significantly higher,and the level of miR-486-5p was significantly lower in the study group than the control group(P＜0.05).Pearson correlation analysis showed that miR-486-5p was negatively correlated with lncRNA PVT1 level in the study group(r=-0.203,P＜0.05).The expression level of lncRNA PVT1 in the transfection group was significantly lower than that in non-transfection group,and the expression level of miR-486-5p in the transfection group was significantly higher than that in the non-transfection group(P＜0.05).The cell proliferation rate of the transfection group was significantly lower than that of the non-transfection group(P＜0.05).After knockdown of lncRNA PVT1,HL-60 cells were significantly blocked in the G1 phase and the apoptosis was induced(P＜0.05).Compared with the non-transfection group,the expressions of Cyclin D1 and CDK4PCNA were significantly downregulated,while the expressions of P21 and P27 were significantly upregulated(P＜0.05).Conclusion Knockdown of lncRNA PVT1 can inhibit proliferation and induce apoptosis in HL-60 cells,while overexpression of miR-486-5p can also inhibit proliferation and induce apoptosis of HL-60 cells.Therefore,it is suggested that knockdown of lncRNA PVT1 can upregulate miR-486-5p in AML patients,serving as a research direction for the prevention and treatment of AML.