LncRNA DNM3OS regulates proliferation and invasion of hepatocellular carcinoma cells via targeting miR-134

SHI Chengyu; FAN Wei; WANG Tingting

doi:10.3969/j.issn.1002-7386.2025.09.009

Abstract：Objective To investigate the impacts of long non-coding RNA DNM3OS(LncRNA DNM3OS)on the proliferation and invasion of hepatocellular carcinoma cells via targeting microRNA-134(miR-134).Methods The quantitative real-time polymerase chain reaction(qRT-PCR)was applied to detect the expression levels of lncRNA DNM3OS and miR-134 in different human hepatocellular carcinoma cells.HepG2 cells were induced with blank control,or transfected with si-DNM3OS,si-NC,si-DNM3OS+miR-NC,and si-DNM3OS+miR-134 inhibitor.After 48 hours of transfection,qRT-PCR was applied to detect the expression levels of lncRNA DNM3OS and miR-134 in cells.The proliferative activity was determined by cell counting kit-8(CCK-8)assay and clony formation assay.Cell invasion was measured by Transwell assay.Apoptosis was analyzed by flow cytometry.The protein expressions of epithelial mesenchymal transition(EMT)-related proteins were detected by Western blot.Dual luciferase reporter assay was used to detect the interaction between LncRNA DNM3OS and miR-134.Results Compared with those induced with blank control,HepG2 cells transfected with si-DNM3OS or si-DNM3OS+miR-NC had significantly lower expression level of DNM3OS,cell survival rate,number of invading cells,and protein expressions of Vimentin and N-cadherin(P＜0.05),but significantly higher apoptotic rate and protein expressions of miR-134 and E-cadherin(P＜0.05).Gain-of-function experiment showed that knockdown of miR-134 partially reversed the regulatory effect of lncRNA DNM3OS knockdown on the proliferation and invasion of hepatocellular carcinoma cells(P＜0.05).MiR-134 was negatively targeted by lncRNA DNM3OS in HepG2 cells(P＜0.05).Conclusion Knockdown of lncRNA DNM3OS can target and upregulate miR-134 to inhibit the proliferation and invasion of hepatocellular carcinoma cells.