Effects of circular RNA hsa_circ_0001982 on the proliferation and apoptosis of osteosarcoma cells by regulating Bcl-2 through miR-143
LIN Bochuan
WANG Dayong
NAN Jing
Abstract：Objective To investigate the effect of circular RNA hsa_circ_0001982 on the proliferation and apoptosis of osteosarcoma cells by regulating B cell lymphoma-2(Bcl-2)through microRNA-143(miR-143).Methods Quantitative reverse transcriptase polymerase chain reaction(qRT-PCR)method was applied to detect the levels of hsa_circ_0001982 and miR-143 in osteosarcoma tissues and cells.Dual-luciferase reporter assay was applied to detect the targeting relationship between hsa_circ_0001982 and miR-143,and that between Bcl-2 and miR-143.U2OS cells were treated with blank control,or transfected with si-NC,si-0001982,si-0001982+anti NC,si-0001982+anti miR-143.Cell proliferation and apoptosis were examined by colony formation assay and the commercial apoptosis assay kit,respectively.Western blot was applied to detect the protein expressions of Ki67,Bcl-2-associated X protein(Bax),and Bcl-2.The in vivo effect of hsa_circ_0001982 on miR-143 and Bcl-2 was examined in a nude mouse xenograft model.Results Hsa_circ_0001982 was significantly upregulated and miR-143 was significantly downregulated in osteosarcoma tissues and cells(P＜0.05).Bioinformatics and dual-luciferase reporter assay showed targeted binding sites between hsa_circ_0001982 and miR-143,and that between Bcl-2 and miR-143.Compared with those transfected with si-NC,U2OS cells transfected with si-0001982 showed significantly lower expression level of has_circ_0001982,colony number,and expression levels of Ki67 and Bcl-2,but higher levels of miR-143,Bax and apoptotic rate(P＜0.05).Compared with those transfected with si-0001982+anti-NC group,miR-143,U2OS cells transfected with si-0001982+anti-miR-143 showed significantly higher colony number,and expression levels of Ki67 and Bcl-2,but lower levels of miR-143,Bax and apoptotic rate(P＜0.05).In the nude mouse model,mice transfected with si-0001982 had significantly lower tumor volume and weight,lower expression levels of hsa_circ_0001982 and Bcl-2 was downregulated,and higher expression of miR-143 than those transfected with si-NC(P＜0.05).Conclusion The hsa_circ_0001982/miR-143/Bcl-2 axis is involved in the occurrence and malignant progression of osteosarcomas.
Keywords：osteosarcomacircular RNA hsa_circ_0001982micro RNA-143B cell lymphoma-2proliferationapoptosis
Publication Date：2025-09-26
Online Publishing Date：2025-10-24(First online date of this platform, not the publication date of the document)
Pages：6( 1450-1455 )