Serum levels of exosome miRNA-126 in patient with metabolic-associated fatty liver disease complicated with ischemic stroke and its clinical diagnostic value
HAN Xue
MAO Xuan
CHENG Jie
Abstract：Objective To analyze the serum levels of exosome miRNA-126 in patient with metabolic-associated fatty liver disease(MAFLD)complicated with ischemic stroke(IS),and to evaluate its clinical diagnostic value.Methods A total of 120 MAFLD patients admitted from January 2022 to December 2023 were selected as the study subjects,including 54 combined with IS as the observation group,and the remaining 66 patients with MAFLD alone were selected as the MAFLD group.In addition,50 healthy subjects were selected as control group.Serum level of exosome miRNA-126 was detected by reverse transcription polymerase chain reaction(RT-PCR).The general clinical indicators and serum level of exosome miRNA-126 were compared.The influencing factors of miRNA-126 were analyzed by multivariate linear stepwise regression,and the influencing factors of MAFLD combined with IS were analyzed by binary logistic regression,so as to evaluate the diagnostic efficacy of serum level of exosome miRNA-126 in MAFLD combined with IS.Results Serum level of exosome miRNA-126 in MAFLD group and observation group was significantly lower than that of the control group,which was significantly lower in the observation group than the MAFLD group(P＜0.05).Compared to baselines before treatment,serum level of exosome miRNA-126 in the MAFLD group and observation group after treatment was significantly higher(P＜0.05),but comparable in the control group(P＞0.05).Multivariate linear stepwise regression analysis showed that age,body mass index(BMI),triglyceride(TG)and total cholesterol(TC)significantly influenced serum level of exosome miRNA-126(P＜0.05).Logistic regression analysis showed that BMI and TG were independent risk factors for MAFLD combined with IS(P＜0.05),while exosome miRNA-126 was a protective factor for it(P＜0.05).The receiver operating characteristic(ROC)curve analysis showed that the optimal cut-off of serum level of exosome miRNA-126 in distinguishing MALFD combined with IS was 3.12,with the sensitivity of 65.43％,specificity of 90.12％and area under the curve(AUC)of 0.890.Conclusion Decreased serum level of exosome miRNA-126 is a risk factor for MAFLD complicated with IS,showing a good diagnostic efficacy on it.
Keywords：metabolism-related fatty liver diseaseischemic strokemiRNA-126diagnostic value
Publication Date：2025-09-26
Online Publishing Date：2025-10-24(First online date of this platform, not the publication date of the document)
Pages：5( 1431-1434,1439 )