Expression levels of MMP-2 and TIMP-1 in children with Henoch-Schonlein purpura and their significances

SHAN Guimei; DONG Chen; GUAN Fengjun

doi:10.3969/j.issn.1002-7386.2025.08.006

Abstract：Objective To investigate the expression levels of matrix metalloproteinase 2(MMP-2)and tissue inhibitor of metalloproteinase 1(TIMP-1)in serum of Henoch-Schonlein purpura(HSP)children and their significances.Methods A total of 71 children with HSP admitted to the Pediatrics Department of the Affiliated Hospital of Xuzhou Medical University from June 2022 to July 2023 were selected.Based on their renal involvement,34 children without renal involvement were included in the HSP group,37 children with renal involvement were include in the Henoch-Schonlein purpura nephritis(HSPN)group.Furthermore,26 healthy children from the same period were chosen as healthy control group.Enzyme-linked immunosorbent assay(ELISA)was adopted to measure the serum levels of MMP-2 and TIMP-1.Their expression levels of each group were compared,and the correlation with clinical and laboratory indicators was analyzed.Results Serum levels of MMP-2 and TIMP-1 in HSPN group and HSP group were significantly higher than those of healthy control group(P＜0.05).Serum MMP-2 and TIMP-1 in HSPN group were significantly higher than those of HSP group(P＜0.05).The interleukin-6(IL-6),tumor necrosis factor alpha(TNF-α),urinary albumin-to-creatinine ratio(UACR),C-reactive protein(CRP)in HSPN group were significantly higher than those of HSP group and healthy control group(P＜0.05).IL-6,TNF-α and CRP levels in HSP group were significantly higher than those of healthy control group(P＜0.05).There was no significant difference in UACR level between HSP group and healthy control group(P＞0.05).Correlation analysis showed that serum MMP-2 level was positively correlated with IL-6,TNF-α,UACR and CRP levels.Conclusion MMP-2 and TIMP-1 are involved in the occurrence of HSP in children,and the serum level of MMP-2 is significantly correlated with the degree of kidney damage.