Quercetin alleviates polycystic ovary syndrome with insulin resistance in rats by mediating advanced glycosylation end products
Adilijiang Wusiman
BIAN Haitao
Shajidai Kuerban
Abstract：Objective To investigate the role of quercetin(QUE)in ameliorating polycystic ovary syndrome with insulin resistance(PCOS-IR)in rats by regulating the advanced glycosylation end products(AGEs).Methods Female Sprague-Dawley(SD)rats in 6 weeks old were randomly divided into the control group,PCOS-IR group,low-dose QUE(QUE-L)group,medium-dose QUE(QUE-M)group,high-dose QUE(QUE-H)group and metformin hydrochloride(MH)group,with 10 rats in each group.Except for those in the control group,rats in the remaining groups were subjected to PCOS-IR modeling by feeding high-fat and high-sugar diet combined with gavage of letrozole sodium carboxymethyl cellulose suspension.After modeling,QUE at varied doses and MH were given by gavage according to the groups.The hematoxylin and eosin(H&E)staining was used to observe the histopathological changes of ovary in rats.Serum levels of testosterone(T),estradiol(E2),luteinizing hormone(LH),follicle stimulating hormone(FSH),fasting insulin(FINS)and AGEs were determined by enzyme-linked immunosorbent assay(ELISA).Fasting plasma glucose(FPG)was detected and the homeostatic model assessment for insulin resistance(HOMA-IR)was calculated.Immunohistochemical staining and Western blot were used to detect the positive expression of AGEs and the receptor for AGE(RAGE)in rat ovarian tissues.Results Compared with rats of PCOS-IR group,rats in the QUE-L group,QUE-M group,QUE-H group and MH group had significantly lower number of follicles in ovarian tissue of rats,more compact structure of ovarian granulosa cells,lower serum T,LH,LH/FSH,FPG,FINS,HOMA-IR,positive expression rates of AGEs and RAGE in ovarian tissues,protein levels of AGEs and RAGE,but higher E2 and FSH(P＜0.05).Serum AGEs were significantly lower in rats of QUE-M,QUE-H and MH groups than the PCOS-IR group(P＜0.05).Conclusion Quercetin can inhibit glycosylation and improve the insulin resistance in rats with PCOS-IR by inhibiting AGEs and RAGE.
Keywords：quercetinpolycystic ovary syndrome with insulin resistanceovarian tissueglycosylation end products
Publication Date：2025-08-26
Online Publishing Date：2025-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 1237-1242 )