Impacts of LINC01106 on the migration,invasion and proliferation of liver cancer cells via the miR-372-3p/SMYD2 axis
HU Zhongzhuo
LIU Hongying
Abstract：Objective To investigate the impacts of long non-coding RNA 01106(LINC01106)on the proliferation,migration,and invasion of liver cancer cells by regulating the microRNA-372-3p(miR-372-3p)/SET and MYND domain containing protein 2(SMYD2)axis.Methods Real-time fluorescence quantitative PCR(qRT-PCR)was applied to detect the expression levels of LINC01106,miR-372-3p,and SMYD2 in liver cancer tissues and cell lines(Huh-7,HepG2,SMMC-7721).SMMC-7721 cells were induced with blank control,or transfected with si-LINC01106,si-NC,si-LINC01106+miR-372-3p inhibitor or si-LINC01106+inhibitor-NC,followed by measurements of LINC01106,miR-372-3p and SMYD2 levels.Cell proliferation was detected by MTT assay and EdU staining.Cell migration and invasion were examined by wound healing assay and Transwell assay,respectively.Western blot(WB)was applied to detect the protein expressions of PCNA,MMP-2,MMP-9,and SMYD2.The relationship between LINC01106 or SMYD2 and miR-372-3p was confirmed by dual luciferase reporter assay.Results LINC01106 and SMYD2 were significantly up-regulated,and miR-372-3p was significantly down-regulated in liver cancer tissues and cell lines(P＜0.05).Compared with those of blank control or transfected with si-NC,SMMC-7721 cells transfected with si-LINC01106 had significantly lower mRNA levels of LINC01106 and SMYD2,OD490 value,EdU-positive cell ratio,wound healing ratio,invasive cell number and protein levels of PCNA,MMP-9,MMP-2 and SMYD2,but higher expression level of miR-372-3p(P＜0.05).Compared with those transfected with si-LINC01106+inhibitor-NC or si-LINC01106,SMMC-7721 cells transfected with si-LINC01106+miR-372-3p inhibitor had significantly higher mRNA levels of LINC01106 and SMYD2,OD490 value,EdU-positive cell ratio,wound healing ratio,invasive cell number and protein levels of PCNA,MMP-9,MMP-2 and SMYD2,but lower expression level of miR-372-3p(P＜0.05).LINC01106 targeted a negative regulation of miR-372-3p,while miR-372-3p targeted a negative regulation of SMYD2.Conclusion Knockdown of LINC01106 inhibits migration,invasion,and proliferation of SMMC-7721 cells by targeting the miR-372-3p/SMYD2 axis.
Keywords：long non-coding RNA01106micro RNA-372-3pSET and MYND domain containing protein 2liver cancerproliferationmigrationinvasion
Publication Date：2025-05-26
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 721-727 )