Effect of STAT4-mediated circ_0001879 on proliferation and apoptosis of retinal pigment epithelial cell induced by high glucose
LI Xiaojun
GONG Lijun
LIANG Chun
Abstract：Objective To analyze the role of signal transducer and activator of transcription 4(STAT4)in high-glucose induced proliferation and apoptosis of retinal pigment epithelial cells via mediating circ_0001879.Methods adult retinal pigment epithelial cell line-19(ARPE-19)cells cultured in vitro were treated with blank control,normal glucose and high glucose.High glucose-induced cells were further transfected with a series of plasmids.Expression levels of circ_0001879,miRNA-338 and STAT4 were detected in ARPE-19 cells.Cell proliferation and apoptosis were examined.Results Overexpression of miR-338 in high-glucose-induced ARPE-19 cells at 48h and 72h significantly decreased the proliferative rate but increased apoptotic rate(P＜0.05).Knockdown of miR-338 in high-glucose-induced ARPE-19 cells at 48h and 72h significantly increased the proliferative rate but decreased apoptotic rate(P＜0.05).Dual-luciferase reporter assay showed that co-transfection of miR-338 mimics and WT-circ_0001879 significantly decreased the luciferase activity than those co-transfected with miR-NC and WT-circ_0001879(P＜0.05).Knockdown of miR-338 in high-glucose-induced ARPE-19 cells at 24 h,48h and 72h significantly increased the proliferative rate but decreased apoptotic rate(P＜0.05).Compared with those co-transfected with miR-inhibitor and si-NC,co-transfection of miR-inhibitor and si-circ_0001879 at 24 h,48h and 72h significantly decreased the proliferative rate but increased apoptotic rate(P＜0.05).Knockdown of STAT4 by transfection of si-STAT4 significantly downregulated circ_0001879(P＜0.05).Overexpression of STAT4 by transfection of pcDNA3.1-STAT4 significantly upregulated circ_0001879(P＜0.05).Conclusion STAT4 promotes proliferation and inhibits apoptosis of ARPE-19 cells by activating circ_0001879.Knockdown of STAT4 inhibits proliferation and stimulates apoptosis of ARPE-19 cells.
Keywords：high glucose inductionretinal pigment epithelial cellssignal transducer and activator of transcription 4(STAT4)circ_0001879proliferationapoptosis
Publication Date：2025-04-26
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 555-559 )