Molecular mechanism of allicin in inhibiting inflammatory factors in rats with intracerebral hemorrhage by regulating miR-125b-5p
GUO Hongsheng
WANG Xianming
LI Yongzhao
Abstract：Objective To explore the effect of allicin on regulating inflammatory factors in rats with intracerebral hemorrhage and its molecular mechanism.Methods The rat model of intracerebral hemorrhage(ICH)was prepared by collagenase method.ICH rats were randomly divided into model group,low-dose,medium-dose and high-dose allicin groups(15,30,60mg/kg),anti-miR-125b-5p group,anti-miR-con group,anti-miR-125b-5p+allicin group,anti-miR-con+allicin group,and sham operation group.After 24 hours of intraperitoneal injection,the neurological function of rats was evaluated.Quantitative real-time polymerase chain reaction(QRT-PCR)was used to detect the mRNA expressions of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β)and miR-125b-5p in rat brain tissue.Western blot was used to detect the protein expressions of TNF-α,IL-6,IL-1β in rat brain tissue.Results Compared with the sham operation group,ICH rats in the model group had significantly higher neurological function score and mRNA and protein levels of TNF-α,IL-6,and IL-1β,but lower mRNA level of miR-125b-5p(P＜0.05).Compared with the model group,ICH rats in the low-dose,medium-dose and high-dose allicin groups had significantly lower neurological function score and mRNA and protein levels of TNF-α,IL-6,and IL-1β,but higher mRNA level of miR-125b-5p(P＜0.05).Compared with the anti-miR-con group,ICH rats in the anti-miR-125b-5p group had significantly lower mRNA level of miR-125b-5p,but higher mRNA and protein levels of TNF-α,IL-6,and IL-1β in brain tissue(P＜0.05).Compared with the anti-miR-con+allicin group,ICH rats in the anti-miR-125b-5p+allicin group had significantly higher neurological function score and mRNA and protein levels of TNF-α,IL-6,and IL-1β mRNA(P＜0.05).Conclusion Allicin may inhibit the release of inflammatory factors in ICH rats by up-regulating miR-125b-5p,and thus improves neurological deficits after ICH.
Keywords：allicincerebral hemorrhagemiR-125b-5pinflammatory factors
Publication Date：2025-03-26
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 401-405 )