Regulatory effects of circ_0004390 on the proliferation and apoptosis of oral squamous cell carcinoma
LI Wei
MENG Yun
LI Ling
Abstract：Objective To investigate the regulatory effects of circ_0004390 on the proliferation,apoptosis and cisplatin sensitivity of oral squamous cell carcinoma(OSCC)and the underlying mechanism.Methods Expression level of circ_0004390 in OSCC tissue and cells was examined by quantitative real-time PCR(qRT-PCR).Cell proliferation was detected by cell counting kit-8(CCK-8)assay,colony formation assay and EdU(5-ethynyl-2'-deoxyuridine)assay.Apoptosis and mitochondrial membrane potential(MMP)were examined by flow cytometry.Apoptosis-associated proteins were measured by Western blot.Cholecystokinin-8(CCK-8)assay was further conducted to test the influence of cisplatin at various concentrations on cell proliferation.The potential target miRNAs and downstream of circ_0004390 were predicted by the starBase,and their target relationship was verified by dual-luciferase reporter assay.Results Circ_0004390 was highly expressed in OSCC(P＜0.05).Transfection of si-circ_0004390 significantly reduced proliferative ability,colony formation ability,EdU-positive rate,MMP,and protein levels of anti-apoptotic Bcl-2 and Survivin,but elevated apoptotic rate,protein levels of pro-apoptotic Bax,cleaved-PAPR and cleaved-caspase-3,and sensitivity to cisplatin(P＜0.05).Overexpression of circ_0004390 yielded the opposite results.Mechanically,circ_0004390 bound to miR-196b-5p,and HMGA2(high mobility group AT-hook protein)was the downstream target of miR-196b-5p.Conclusion Knockdown of circ_0004390 inhibits proliferation,induces apoptosis and enhances cisplatin sensitivity in OSCC by regulating the downstream miR-196b-5p/HMGA2 axis.
Keywords：oral squamous cell carcinomacirc_0004390miR-196b-5pHMGA2proliferationapoptosis
Publication Date：2025-03-26
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 368-374 )