Impact of circSATB2 on malignant biological behaviors of non-small cell lung cancer cells by regulating miR-150-5p/PDCD4 axis
MAO Wei
GUO Ling
Abstract：Objective To investigate the impact of circular RNA(circRNA)SATB2 on the malignant biological behaviors of non-small cell lung cancer(NSCLC)cells by regulating the miR-150-5 p/programmed cell death 4(PDCD4)axis.Methods The expressions of CircSATB2,miR-150-5p and PDCD4 proteins in NSCLC tissues and paracancerous tissues were detected by quantitative real time polymerase chain reaction(qRT-PCR)and immunohistochemistry.Their expressions in human normal pulmonary epithelial cell line BEAS-2B and NSCLC cell lines HCC827,H1299 and A549 were detected by qRT-PCR and Western blot.A549 cells were treated with blank control,or transfected with circSATB2 overexpression plasmid,circSATB2 knockdown plasmid,circSATB2 negative control(NC)plasmid,miR-150-5p NC,miR-150-5p mimic,circSATB2 knockdown plasmid+inhibitor NC,and circSATB2 knockdown plasmid+miR-150-5p inhibitor.The expression of CircSATB2 and miR-150-5p was detected by qRT-PCR.Cell proliferation was detected by cell counting kit-8(CCK-8)assay.Apoptosis was detected by flow cytometry.Cell migration was detected by wound healing assay.Cell invasion was detected by Transwell assay.Western blot was used to detect the protein expressions of PDCD4,proliferating cell nuclear antigen(PCNA),Bcl-2 associated X protein(Bax),matrix metalloproteinase(MMP-2)and MMP-9.The relationship between CircSATB2 and miR-150-5p/PDCD4 was verified.Results A549 cells were selected as the in vitro model.Knockdown of circSATB2 significantly downregulated circSATB2 and PDCD4,but upregulated miR-150-5p(P＜0.05).Overexpression of miR-150-5p significantly downregulated PDCD4 and upregulated miR-150-5p(P＜0.05).Co-knockdown of circSATB2 and miR-150-5p significantly downregulated miR-150-5p and upregulated PDCD4 than A549 cells with knockdown of circSATB2(P＜0.05).Knockdown of circSATB2 or overexpression of miR-150-5p inhibited proliferation,invasion and migration of A549 cells,but stimulated cell apoptosis.Knockdown of miR-150-5p weakened the regulatory effect of circSATB2 on the proliferation,invasion,migration and apoptosis of A549 cells.CircSATB2 targeted the miR-150-5p/PDCD4 axis.Conclusion Knockdown of circSATB2 inhibits the proliferation,migration and invasion,and enhance the apoptosis of A549 cells by regulating the miR-150-5p/PDCD4 axis.
Keywords：cyclic RNA SATB2miR-150-5pprogrammed cell death 4non small cell lung cancerproliferation
Publication Date：2025-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 181-187 )