Effect of Yuye Decoction on improving GLP-1 in Type 2 Diabetes Mellitus rats by regulating ferroptosis in ileum tissue
CHEN Shifei
GONG Fan
TAO Huilin
Abstract：Objective To observe the effect of Yuye Decoction on improving GLP-1 in type 2 diabetes mellitus(T2DM)rats by regulating ferroptosis in ileum tissue.Methods T2DM rat model was prepared by high fat diet combined with intraperitoneal injection of streptozotocin(STZ).Rats were randomly assigned into model group,high dose group and low dose group,with 8 rats in each group.Eight age-matched rats were included in the control group.After 4 weeks of continuous intragastric administration,oral glucose tolerance tests(OGTT)were carried out,and the area under the curve(AUC)was calculated.Fasting insulin(FINS)was measured.Homeostatic model assessment for β-cell function(HOMA-β),homeostasis model assessment for insulin resistance(HOMA-IR)were calculated.Fe2+,malondialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase 4(GPX4),ferroptosis suppressor protein 1(FSP1)and glucagon-like peptide-1(GLP-1)in rat ileum tissues was detected.Results Compared with those of the model group,rats in the high dose group and low dose group had significantly decreased blood glucose level,AUC,HOMA-IR,Fe2+and MDA(P＜0.05),but increased FINS,HOMA-β,SOD,GPX4,FSP1 and GLP-1levels(P＜0.05).Conclusion Yuye Decoction could ameliorate ferroptosis in ileum tissue of T2DM rats,upregulate GLP-1 and reduce insulin resistance.
Keywords：Yuye Decoctiondiabetes mellitustype 2ferroptosisinsulin resistanceglucagon-like peptide-1
Publication Date：2025-01-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 38-41,46 )