LncRNA EBLN3P mediates the proliferation,apoptosis and migration of breast cancer cells by regulating the miR-202-5p/BAG3 axis
MA Ye
QI Jie
CHEN Jianhua
Abstract：Objective To investigate the effect of long non-coding RNA endogenous Bonavirus like nuclear protein 3 pseudogene(lncRNA EBLN3P)on the proliferation,apoptosis and migration of breast cancer cells and its mechanism.Methods Breast cancer cell lines MDA-MB-468,MDA-MB-231,SK-BR-3 and MCF-7 and human breast epithelial cell line MCF-10A were cultured in vitro.Relative levels of lncRNA EBLN3P and microRNA-202-5p(miR-202-5p)in cell lines were detected by quantitative real-time polymerase chain reaction(qRT-PCR).Protein expression of Bcl2 associated athanogene 3(BAG3)was detected by Western blot.The binding sites of lncRNA EBLN3P and BAG3 with miR-202-5p were predicted by Starbase and validated by dual luciferase reporter assay.MCF-7 cells were induced with blank control,or transfected with si-NC,si-EBLN3P,si-EBLN3P+anti miR-NC,and si-EBLN3P+anti miR-202-5p.qRT-PCR was applied to detect the relative levels of lncRNA EBLN3P,and miR-202-5p.Western blot was applied to detect the protein expression of BAG3.EdU-positive cell ratio,apoptotic rate and wound healing rate were measured by EdU assay,flow cytometry and wound healing assay,respectively.Results Compared with those of MCF-10A cells,lncRNA EBLN3P and BAG3 were significantly upregulated in MDA-MB-468,MDA-MB-231,SK-BR-3,and MCF-7 cells,while miR-202-5p was significantly downregulated(P＜0.05).Bioinformatics analysis showed that miR-202-5p had binding sites with lncRNA EBLN3P and BAG3,and dual luciferase reporter assay confirmed a targeted relationship between the two.Compared with those transfected with si-NC,MCF-7 cells transfected with si-EBLN3P had significantly lower expression levels of lncRNA EBLN3P and BAG3,EdU-positive cell ratio and wound healing rate,but significantly higher level of miR-202-5p and apoptotic rate(P＜0.05).Compared with those transfected with si-EBLN3P+anti-miR-NC,MCF-7 cells transfected with si-EBLN3P+anti-miR-202-5p had significantly higher expression levels of lncRNA EBLN3P and BAG3,EdU-positive cell ratio and wound healing rate,but significantly lower level of miR-202-5p and apoptotic rate(P＜0.05).Conclusion Knockdown of lncRNA EBLN3P inhibits the proliferation and migration of breast cancer cells and promotes cell apoptosis by up-regulating miR-202-5p and downregulating BAG3.
Keywords：breast cancerlong non-coding RNA endogenous Bonavirus like nuclear protein 3 pseudogenemicro RNA-202-5pBcl2 associated athanogene 3proliferationapoptosismigration
Publication Date：2025-01-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 27-32 )