Effects of midazolam on the proliferation,migration,and epithelial mesenchymal transition of colorectal cancer cells by regulating the Notch/Snail signaling pathway
WEN Zhu
LI Jun
Abstract：Objective To investigate the effects of midazolam(Mid)on the proliferation,migration,and epithelial mesenchymal transition(EMT)of colorectal cancer(CRC)cells HT29 and its regulatory effect on the Notch/zinc finger protein transcription factor(Snail)signaling pathway.Methods HT29 cells were cultured in vitro and treated with 0-20μmol/L Mid to select the optimal concentration.HT29 cells were treated with blank control,low-dose(5μmol/L),medium-dose(10μmol/L)and high-dose Mid(20 µmol/L),and high-dose Mid+Notch activator VPA-d4(20μmol/L Mid+50μmol/L VPA-d4).Cell proliferation,migration,invasion and apoptosis were measured by colony formation assay,wound healing assay,Transwell assay and flow cytometry,respectively.Immunofluorescence assay was applied to detect the protein expressions of N-cadherin and E-cadherin.Western blot was applied to detect the protein expressions of Notch 1 and Snail.Results Compared with those of blank control,Mid treatment significantly decreased colony number,migratory rate,invasive cell number and protein expressions of Notch 1,Snail and N-cadherin,but increased apoptotic rate and protein level of E-cadherin in a dose-dependent manner(P＜0.05).Compared with those induced with high-dose Mid,treatment of high-dose Mid+VPA-d4 significantly increased colony number,migratory rate,invasive cell number and protein expressions of Notch 1,Snail and N-cadherin,but decreased apoptotic rate and protein level of E-cadherin(P＜0.05).Conclusion Mid may inhibit the proliferation,migration,and EMT of CRC cells by inhibiting the Notch/Snail signaling pathway.
Keywords：midazolamcolorectal cancer cellsNotch/zinc finger protein transcription factor(Snail)signaling pathwayproliferationmigration
Publication Date：2025-01-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 11-16 )