LncRNA-H19 regulates the progression of B-cell acute lymphoblastic leukemia through the miR-29a-3p/Fzd4 axis
SHEN Wencui
LIU Chang
ZHANG Hongli
Abstract：Objective This study aims to detect the effect of long non-coding RNA H19(lncRNA-H19)on B-cell acute lymphoblastic leukemia(B-ALL)and the underlying mechanism.Methods Expression levels of lncRNA-H19 and miR-29a-3p in B-ALL samples were analyzed using Gene Expression Profiling Interactive Analysis(GEPIA)and Gene Expression Omnibus(GEO)databases,which were verified in NALM-6 and BALL-1 cells by the quantitative polymerase chain reaction(qPCR).After knockdown of lncRNA-H19 or overexpression of miR-29a-3p by cell transfection,proliferation and apoptosis were evaluated by CCK-8 assay and flow cytometry,respectively.Results LncRNA-H19 was significantly upregulated(P＜0.05),while miR-29a-3p was significantly downregulated in B-ALL samples(P＜0.05).Knockdown of lncRNA-H19 or overexpression of miR-29a-3p inhibited the proliferation(P＜0.05)and induced apoptosis of NALM-6 and BALL-1 cells(P＜0.01).MiR-29a-3p was found to directly target Fzd4,showing a negative correlation in B-ALL samples from online databases.Furthermore,lncRNA-H19 was found to regulate the Wnt/β-catenin pathway through the miR-29a-3p/Fzd4 axis.Conclusion LncRNA-H19 is upregulated in B-ALL cases and mediates the growth and apoptosis of B-ALL cells.It regulates the Wnt/β-catenin pathway through the miR-29a-3p/Fzd4 axis.Our findings provide new insights into the role of lncRNA-H19 in the pathogenesis of B-ALL and therapeutic strategies.
Keywords：B-cell leukemialong non-coding RNA H19(lncRNA-H19)miR-29a-3pFzd4
Publication Date：2025-01-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 5-10 )