Intervention effect of Qingyi Decoction in rats with severe acute pancreatitis through regulating the PI3K/AKT signaling pathway
WANG Yang
LIU Xinbo
WANG Zeyu
Abstract：Objective To analyze the efficacy of Qingyi Decoction(pancreas clearing decoction)in rats with severe acute pancreatitis through the regulation of phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)signaling.Methods Forty-eight healthy Sprague-Dawley(SD)rats in the specific pathogen-free(SPF)level were selected.Eight rats were randomly selected in the control group(n=4)and sham group(n=4),and 32 rats were selected for severe acute pancreatitis model.Among them,32 rats were successfully modelled,and randomly divided into model group(n=16)and Qingyi Decoction group(n=16).The pathological histology,amylase,inflammatory cytokines,blood urea nitrogen(BUN),creatinine(Cr),superoxide dismutase(SOD),malondialdehyde(MDA),expression levels of proteins in the PI3K/AKT pathway were observed in each group.Results Compared with those of control group,rats in the sham group,model group and Qingyi Decoction group presented significantly higher serum amylase,BUN and Cr at 6h and 12h(P＜0.05).Compared with those of sham group,rats in the model group presented significantly higher serum amylase,BUN and Cr at 6h and 12h(P＜0.05).Compared with those of sham group,rats in the Qingyi Decoction group presented significantly lower serum amylase,BUN and Cr at 6h and 12h(P＜0.05).Compared with those of model group,rats in the Qingyi Decoction group presented significantly lower serum amylase,BUN and Cr at 6h and 12h(P＜0.05).Compared with those of control group,rats in the sham group,model group and Qingyi Decoction group presented significantly higher TNF-α,IL-6 and MDA at 6h and 12h,but lower IL-4 and SOD levels at 6h and 12h(P＜0.05).Compared with those of sham group,rats in the model group presented significantly higher TNF-α,IL-6 and MDA at 6h and 12h,but lower IL-4 and SOD levels at 6h and 12h(P＜0.05).Compared with those of sham group,rats in the Qingyi Decoction group presented significantly lower TNF-α,IL-6 and MDA at 6h and 12h,but higher IL-4 and SOD levels at 6h and 12h(P＜0.05).Compared with those of model group,rats in the Qingyi Decoction group presented significantly lower TNF-α,IL-6 and MDA at 6h and 12h,but higher IL-4 and SOD levels at 6h and 12h(P＜0.05).Compared with those of control group,rats in the sham group,model group and Qingyi Decoction group presented significantly higher mRNA and protein levels of PI3K and AKT(P＜0.05).Compared with those of sham group,rats in the model group and Qingyi Decoction group presented significantly higher mRNA and protein levels of PI3K and AKT(P＜0.05).Compared with those of model group,rats in the Qingyi Decoction group presented significantly lower mRNA and protein levels of PI3K and AKT(P＜0.05).Conclusion The intervention of Qingyi Decoction in severe acute pancreatitis model rats can improve the pathological histology and reduce inflammation,blood urea nitrogen and creatinine by regulating the PI3K/AKT signaling pathway.
Keywords：severe acute pancreatitisqingyi decoctionphosphatidylinositol 3-kinaseprotein kinase bintervention effectsinflammatory mediators
Publication Date：2024-12-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 3707-3711 )