Effects of glycyrrhizin on the proliferation,apoptosis,and invasion of hepatocellular carcinoma cells by regulating the Notch signaling pathway
BU Xiaofen
LI Jun
ZHU Hong
Abstract：Objective To investigate the effect of glycyrrhizin(GL)on the proliferation,apoptosis,and invasion of hepatocellular carcinoma cells by regulating the Notch signaling pathway.Methods Hep3B cells in a good growth state were taken and treated with different concentrations of GL and Notch signaling pathway activators and inhibitors.They were treated with low-dose(GL-L,1 mmol/L GL),medium-dose(GL-M,2mmol/L GL)and high-dose GL(GL-H,4mmol/L GL),GL-H+Notch activator(4mmol/L GL+5mg/L Jagged 1)and GL-H+Notch inhibitor(4 mmol/L GL+10 μmol/L DAPT).The proliferation,invasion,migration,apoptosis of Hep3B cells were detected by MTT assay,Transwell assay,scratch assay,flow cytometry,respectively.Protein expressions of Cyclin D1,Bax,Notch1,Hes1,Snail,E-cadherin were detected by Western blot.Results Compared with those of blank control,Hep3B cells induced with GL-L,GL-M and GL-H presented significantly lower proliferative rate,invasive number,migratory rate and protein levels of Cyclin D1,Notch1,Hes1 and Snail,but higher apoptotic rate and protein levels of Bax and E-cadherin(P＜0.05).Compared with those induced with GL-H,Hep3B cells induced with GL-H+Notch activator presented significantly higher proliferative rate,invasive number,migratory rate and protein levels of Cyclin D1,Notch1,Hes1 and Snail,but lower apoptotic rate and protein levels of Bax and E-cadherin(P＜0.05).Compared with those induced with GL-H,Hep3B cells induced with GL-H+Notch inhibitor presented significantly lower proliferative rate,invasive number,migratory rate and protein levels of Cyclin D1,Notch1,Hes1 and Snail,but higher apoptotic rate and protein levels of Bax and E-cadherin(P＜0.05).Conclusion GL inhibits the proliferation,invasion,and migration of hepatocellular carcinoma cells and promote their apoptosis by inhibiting the Notch signaling pathway.
Keywords：glycyrrhizinNotch signaling pathwayproliferationapoptosisinvasion
Publication Date：2024-11-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 3370-3374 )