Correlation of serum miR-92a-3p and miR-223-3p levels with clinical prognosis of traumatic fractures

XIAO Jinqi; WANG Xufang; LIU Youning

doi:10.3969/j.issn.1002-7386.2024.21.008

Abstract：Objective To investigate the correlation of serum miR-92a-3p and miR-223-3p levels with clinical prognosis of traumatic fractures.Methods From August 2021 to August 2022,112 patients with traumatic fractures(traumatic fracture group)and 112 healthy volunteers who underwent physical examinations(control group)were included in the study.According to the National Early Warning Score(NEWS),patients with traumatic fractures were grouped into mild-to-moderate group(n=73)and severe group(n=39).According to the outcome after hospitalization,they were separated into the emergency surgery group(n=48),ICU admission group(n=39),and the emergency death group(n=25).Real-time fluorescence quantitative PCR(qRT-PCR)was applied to detect serum levels of miR-92a-3p and miR-223-3p.Spearman correlation was applied to analyze the correlation of serum miR-92a-3p and miR-223-3p with the NEWS score.Multivariate Logistic regression was applied to analyze the influencing factors for the prognosis of traumatic fractures.The receiver operating characteristic(ROC)curve was applied to analyze the predictive value of serum miR-92a-3p and miR-223-3p levels in the prognosis of patients with traumatic fractures.Results Compared with those of the control group,patients in the traumatic fracture group had significantly lower serum miR-92a-3p and higher serum miR-223-3p levels(P＜0.05).Serum miR-92a-3p level in the mild-to-moderate group was significantly higher than that of severe group,while the serum miR-223-3p level was significantly lower than that of severe group(P＜0.05).Serum miR-92a-3p was negatively correlated with the NEWS score(r=-0.692,P＜0.05),while serum miR-223-3p was positively correlated with the NEWS score(r=0.603,P＜0.05).Serum miR-92a-3p levels in patients with traumatic fractures gradually decreased in the emergency surgery group,ICU admission group,and emergency death group(P＜0.05),while miR-223-3p increased significantly in sequence(P＜0.05).According to multivariate Logistic regression analysis,miR-223-3p was a risk factor for the prognosis of patients with traumatic fractures(P＜0.05),while miR-92a-3p was a protective factor(P＜0.05).The area under the ROC curve(AUC)of serum miR-92a-3p combined with miR-223-3p to predict the prognosis of traumatic fracture was 0.824,which was larger than the single detection(Z combination-miR-92a-3P=2.519,P=0.012;Z combination-miR-223-3P=2.321,P=0.020).Conclusion Serum miR-92a-3p level in patients with traumatic fractures decreases,while serum miR-223-3p level increases.They are closely related to the prognosis of traumatic fractures,and their combined detection can better evaluate the prognosis of patients with traumatic fractures.