Effect of quercetin on ovarian structure and function on rats with premature ovarian insufficiency by regulating the SDF-1/CXCR4 signaling axis
GUO Qian
MA Weirong
WANG Haidan
Abstract：Objective To investigate the effect of quercetin on the ovarian structure and function in rats with premature ovarian insufficiency(POI)by regulating the stromal cell-derived factor-1(SDF-1)/CXC chemokine receptor 4(CXCR4)signaling pathway.Methods A POI rat model was constructed by injecting cyclophosphamide.Rats were randomly grouped into control group,model group,low-dose quercetin group(25.0mg/kg),high-dose quercetin group(100mg/kg),and high-dose quercetin+CTCE-0214 group(100mg/kg quercetin+10.0mg/kg CTCE-0214).Rats in the control group were given the same amount of normal saline.Serum follicle stimulating hormone(FSH),estradiol(E2),anti Mullerian hormone(AMH),superoxide dismutase(SOD),and malondialdehyde(MDA)were measured by Enzyme-linked immunosorbent assay(ELISA).The ovarian index of rats in each group was detected.H&E staining was applied to detect pathological damage in rat ovarian tissue and follicle counting.Immunohistochemical staining was performed to detect the positive expression of ovarian estrogen receptor(ER).Western blot was applied to detect the protein expressions of SDF-1 and CXCR4 in ovarian tissues.Results Compared with those of control group,rats in the model group presented significantly higher serum FSH and MDA levels,number of atretic follicles and protein levels of SDF-1 and CXCR4,but lower serum E2,AMH and SOD levels,ovarian index,number of mature follicles in ovarian tissues and protein level of ER(P＜0.05).Compared with those of model group,rats in the low-dose and high-dose quercetin groups presented significantly lower serum FSH and MDA levels,number of atretic follicles and protein levels of SDF-1 and CXCR4,but higher serum E2,AMH and SOD levels,ovarian index,number of mature follicles in ovarian tissues and protein level of ER(P＜0.05).Rats in the high-dose quercetin+CTCE-0214 group presented significantly aggravated damages in the structure and function of ovaries than those of high-dose quercetin group(P＜0.05).Conclusion Quercetin may exert a protective effect on the ovarian structure and function of premature ovarian insufficiency rats by inhibiting the SDF-1/CXCR4 signaling pathway.
Keywords：quercetinstromal cell-derived factor-1CXC chemokine receptor 4premature ovarian insufficiency
Publication Date：2024-11-10
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 3237-3241 )