CircPARD3 regulates migration,invasion,and epithelial mesenchymal transition of oral squamous cell carcinoma cell by regulating the miR-326/CXCL3 axis
YIN Jiang
TANG Ping
ZHANG Hui
Abstract：Objective To investigate the regulatory effects of cyclic RNA PARD3(circPARD3)on migration,invasion and epithelial mesenchymal transformation(EMT)of oral squamous cell carcinoma(OSCC)cells by targeting the microRNA-326(miR-326)/CXC chemokine ligand 3(CXCL3)axis.Methods Expression levels of circPARD3 and miR-326 in OSCC tissues and cells were detected by quantitative real-time polymerase chain reaction(qRT-PCR).The target relationship between circPARD3 and miR-326,and that between miR-326 and CXCL3 was verified by the dual-luciferase reporter assay.The OSCC cell line CAL-27 was transfected with si-NC,si-circPARD3,si-circPARD3+anti-NC,si-circPARD3+ anti-miR-326,miR-NC,and miR-326 mimics respectively,and CAL-27 cells treated with blank control were set as the control group.Cell proliferation,migration,and invasion were detected by colony formation assay,wound healing assay and Transwell assay,respectively.Western blot was applied to detect the protein expressions of E-cadherin,N-cadherin and Vimentin in CAL-27 cells.An in vivo OSCC xenograft mouse model was created to verify the effects of circPARD3 on OSCC tumor growth and the involvement of the miR-326/CXCL3 axis.Results In OSCC tissues and cells,circPARD3 was up-regulated,while miR-326 was down-regulated.Knockdown of circPARD3 significantly inhibited OSCC cell proliferation,migration,invasion,and EMT,which were significantly reversed by knockdown of miR-326.The in vivo OSCC xenograft mouse model showed that knockdown of circPARD3 significantly decreased the volume and weight of xenograft tumors,and upregulated miR-326 and downregulated CXCL3.Conclusion CircPARD3 is up-regulated in OSCC tissues and cells,and knockdown of circPARD3 inhibits OSCC cell migration,invasion and EMT by up-regulating miR-326,thereby down-regulating CXCL3 expression.
Keywords：circular RNA PARD3micro RNA-326CXC chemokine ligand 3oral squamous cell carcinomamigrationinvasionepithelial mesenchymal transformation
Publication Date：2023-12-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 3697-3702 )