The neuroprotective effects of Gastrodia elata extract on SH-SY5Y cell injury induced by H2O2 in vitro

doi:10.3969/j.issn.1002-7386.2018.10.008

Abstract：Objective To investigate the neuroprotective effects of Gastrodia elata extract on SH-SY5Y cell injury induced by H2O2 in vitro,and to explore its molecular biological mechanism.Methods The SH-SY5Y cells were treated by H2O2 in vitro to induce SH-SY5Y cell injury model,then the Gastrodia elata extract was added into the medium at the concentration of 0,0.1,1,10,100μg/ml,respectively.The cell morphological changes were observed by microscopy,and cell proliferation was detected by MTT,moreover,the expression levels of related genes with nerve cells growth including nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) were detected by qPCR method.Results After 24-hour induction by H2O2,the obvious morphological changes were found in SH-SY5Y cells,with cell synapse length being decreased significantly (P ＜ 0.05),and cell proliferation was obviously decreased (P ＜ 0.05),however,after H2O2 cells were treated by Gastrodia data extracts,the cytotoxicity caused by H2O2 was obviously inhibited,moreover,with the increase of Gastrodia elata extract concentration,the cell synapse length and cell proliferation were gradually improved,furthermore,there were significant differences among different concentrations of Gastrodia elata extracts (P ＜ 0.05).In addition the expression levels of NGF and BDNF in SH-SYSY cells with injury induced by H2O2 were significantly decreased (P ＜ 0.05),however,after the cells were treated by different concentrations of Gastrodia elata extract,the expression levels of NGF and BDNF were significantly upregulated (P ＜0.05).Conclusion Gastrodia elata extract has protective effects on SH-SY5Y cells with injury induced by H2O2,and its action mechanism may be correlated with its regulative effects on the expressions of NGF and BDNF.